The ability to synthesize DNA with high precision is a cornerstone of modern biological research, diagnostics, and therapeutic development. This capability is largely thanks to the phosphoramidite method of DNA synthesis, a highly efficient chemical process. At the heart of this method are specialized reagents like Methyl Tetraisopropylphosphorodiamidite (CAS 92611-10-4). As a leading chemical supplier, NINGBO INNO PHARMCHEM CO.,LTD. is proud to provide this essential component to the scientific community.

How DNA Synthesis Works: The Phosphoramidite Approach

The phosphoramidite method, pioneered in the early 1980s, revolutionized DNA synthesis. It is a cyclical process involving the stepwise addition of protected nucleotide building blocks. Each cycle typically includes four main steps:

  1. Deprotection: The 5'-hydroxyl group of the growing DNA chain, which is protected by a dimethoxytrityl (DMT) group, is deprotected using a mild acid. This exposes the reactive hydroxyl group.
  2. Coupling: A nucleotide phosphoramidite, such as a derivative of Methyl Tetraisopropylphosphorodiamidite, is activated and coupled to the exposed 5'-hydroxyl group. This step forms a phosphite triester linkage. The efficiency of this coupling is critical and directly influenced by the quality of the phosphoramidite reagent used.
  3. Oxidation/Capping: The newly formed phosphite triester linkage is oxidized to a more stable phosphate triester linkage, which forms the DNA backbone. Unreacted 5'-hydroxyl groups are often capped to prevent the formation of deletion sequences.
  4. Reiteration: The cycle repeats, adding one nucleotide at a time, until the desired DNA sequence is assembled.

The Role of Methyl Tetraisopropylphosphorodiamidite

Methyl Tetraisopropylphosphorodiamidite serves as the precursor to the activated nucleotide phosphoramidites used in the coupling step. Its specific chemical structure, featuring bulky isopropyl groups, offers significant advantages:

  • Enhanced Stability: It is less prone to hydrolysis and decomposition compared to many other phosphoramidites, ensuring a longer shelf life and more reliable performance during synthesis. This is a key reason why researchers seek to buy it from reputable manufacturers.
  • Efficient Coupling: The reagent's optimal reactivity ensures rapid and efficient coupling with the growing DNA chain, leading to higher yields and fewer incomplete sequences.
  • High Purity: NINGBO INNO PHARMCHEM CO.,LTD. emphasizes the high purity of this reagent, which is critical for preventing side reactions and ensuring the fidelity of the synthesized DNA.

Applications and Availability

This phosphoramidite is fundamental for synthesizing custom DNA sequences used in a wide range of applications, including PCR primers, sequencing primers, gene synthesis, gene silencing agents (like siRNAs), and components for gene therapy. As a leading manufacturer and supplier, NINGBO INNO PHARMCHEM CO.,LTD. ensures that this vital reagent is available to meet the demands of both academic research and commercial production. We understand the importance of timely delivery and consistent quality, making us a preferred partner for your DNA synthesis needs.

For those looking to purchase Methyl Tetraisopropylphosphorodiamidite, partnering with a trusted supplier like NINGBO INNO PHARMCHEM CO.,LTD. guarantees access to a high-quality product that underpins successful DNA synthesis. Contact us today to inquire about pricing and availability.