Spectrophotometric assays form a cornerstone of modern analytical chemistry and diagnostics, enabling precise quantification of various substances. At the heart of many such assays for hydrogen peroxide and related compounds lies Sodium 3-(N-Ethyl-3-methylanilino)-2-hydroxypropanesulfonic Acid Sodium Salt, widely known as TOOS (CAS: 82692-93-1). As a dedicated manufacturer and supplier of fine chemicals, we illuminate the scientific underpinnings of this crucial reagent.

TOOS functions as a chromogenic substrate in conjunction with 3-methyl-2-benzothiazolinone hydrazone (MBTH). The fundamental principle involves the enzymatic generation of hydrogen peroxide, which then participates in a series of oxidative reactions. In the presence of a peroxidase enzyme, MBTH is oxidized to a reactive intermediate. This intermediate then couples with TOOS to form a stable, colored product – typically a blue-violet quinoid dye. The intensity of this color, directly proportional to the concentration of the analyte that generated hydrogen peroxide, is measured using a spectrophotometer.

This MBTH-TOOS system is highly valued for its sensitivity, specificity, and broad applicability in clinical diagnostics and biochemical research. It is instrumental in assays for enzymes like glucose oxidase, cholesterol oxidase, and lactate oxidase, where the production of hydrogen peroxide is a direct measure of the substrate's concentration. For B2B clients, especially those in the diagnostic kit manufacturing sector, sourcing a reliable and consistently pure TOOS from a reputable supplier is non-negotiable. Our role as a manufacturer ensures that the TOOS we supply meets the stringent requirements for these sensitive applications.

When you consider purchasing TOOS, remember its critical role in assay performance. The quality of the TOOS directly impacts the sensitivity, linearity, and overall reliability of the final diagnostic assay. We, as experienced manufacturers, are committed to providing TOOS that guarantees optimal performance, making your research and product development efforts more efficient and accurate. We invite you to buy from us and experience the difference that high-quality chemical intermediates can make. Contact our sales team for a detailed quote and to learn more about our supply capabilities.