For researchers and procurement specialists in the pharmaceutical and biotechnology sectors, sourcing high-quality chemical intermediates is a critical task that directly impacts project timelines and outcomes. N-Cbz-L-histidine (Z-His-OH) is a prime example of such an intermediate, widely used in peptide synthesis. This article serves as a buyer’s guide to understanding what makes quality Z-His-OH and how to effectively procure it, with a focus on global supply chains and competitive pricing.

Z-His-OH, or N-alpha-Carbobenzyloxy-L-histidine, is a derivative of the amino acid histidine. Histidine is vital for many biological functions due to its imidazole ring, which can participate in acid-base catalysis and metal ion binding. In peptide synthesis, particularly solid-phase peptide synthesis (SPPS), the alpha-amino group of histidine must be protected to ensure controlled chain elongation. The Cbz (benzyloxycarbonyl) group offers robust protection, being stable during coupling reactions and removable via mild hydrogenation, which is compatible with many other protecting groups used in SPPS.

When a buyer is looking to purchase Z-His-OH, several factors are crucial to evaluate. Firstly, purity is paramount. High-purity Z-His-OH (typically >98%) is essential to prevent side reactions and ensure the integrity of the final peptide product. Key physical characteristics to note include its appearance, usually a white to off-white crystalline powder, and its melting point (around 168°C dec.). A supplier’s Certificate of Analysis (CoA) is indispensable for verifying these specifications.

Secondly, cost-effectiveness plays a significant role. Manufacturers and suppliers, particularly those with established production facilities in countries like China, often provide Z-His-OH at a more competitive price point. This allows research institutions and manufacturing companies to secure the necessary quantities for their projects without excessive budget strain. When comparing Z-His-OH price, it’s vital to balance cost with guaranteed quality and reliable supply chain management.

Thirdly, reliability of supply and supplier reputation are key. Buyers should look for manufacturers with a proven track record in producing peptide synthesis reagents. This includes ensuring they can consistently deliver the required quantities and adhere to quality control standards. Engaging with suppliers who offer responsive customer service and technical support can also streamline the procurement process.

The application of Z-His-OH in peptide synthesis is straightforward: it is activated and coupled to the growing peptide chain. After successful coupling, the Cbz group is removed by catalytic hydrogenation. This systematic approach enables the construction of complex peptides for research, diagnostics, and therapeutics. Therefore, ensuring a stable supply of quality Z-His-OH is fundamental to uninterrupted R&D and production cycles.

In conclusion, acquiring Z-His-OH requires careful consideration of purity, price, and supplier reliability. By focusing on these aspects and leveraging the global market for chemical intermediates, buyers can secure this essential building block and advance their peptide synthesis projects with confidence.