For chemists, researchers, and formulation scientists in the B2B sector, rigorous characterization of chemical intermediates is paramount. This ensures product quality, guides synthetic optimization, and confirms identity. In this context, understanding the spectroscopic properties of 2-Hydroxy-6-methyl-5-nitropyridine (CAS No. 28489-45-4) is essential. As a leading manufacturer and supplier of fine chemical intermediates, we highlight the key spectroscopic techniques that provide critical insights into this compound’s structure and purity.

2-Hydroxy-6-methyl-5-nitropyridine is a pyridine derivative featuring a nitro group, a methyl group, and a hydroxyl group. Each of these functional groups, along with the aromatic pyridine ring, contributes unique signatures in various spectroscopic analyses. These techniques are invaluable for quality control, enabling us to confirm that every batch meets stringent specifications, typically exceeding 98.0% purity.

Nuclear Magnetic Resonance (NMR) Spectroscopy: NMR is arguably the most powerful tool for elucidating the structure of organic molecules. Both ¹H NMR and ¹³C NMR spectroscopy provide detailed information about the hydrogen and carbon atoms within the molecule, respectively. For 2-Hydroxy-6-methyl-5-nitropyridine, ¹H NMR will reveal distinct signals for the methyl protons, the aromatic protons on the pyridine ring, and potentially a signal for the hydroxyl or tautomeric amide proton. The chemical shifts and splitting patterns are diagnostic of the substitution pattern. ¹³C NMR will confirm the presence of the unique carbon atoms, including the carbonyl carbon in its pyridone tautomeric form, and the carbons attached to the nitro and methyl groups. Two-dimensional NMR techniques like HSQC and HMBC can further confirm the connectivity of atoms, providing unambiguous structural confirmation.

Infrared (IR) Spectroscopy: IR spectroscopy is excellent for identifying the presence of specific functional groups. For 2-Hydroxy-6-methyl-5-nitropyridine, characteristic absorption bands are expected for: the O-H stretching vibration (often broad due to hydrogen bonding), C-H stretching from the methyl and aromatic groups, C=O stretching (if the pyridone tautomer is present), and the strong asymmetric and symmetric stretching vibrations of the nitro group (typically around 1500-1550 cm⁻¹ and 1300-1350 cm⁻¹). These IR signals serve as a fingerprint for the molecule and are crucial for rapid quality checks.

Mass Spectrometry (MS): Mass spectrometry provides the molecular weight of the compound and can help identify fragmentation patterns that are characteristic of its structure. Techniques like Electrospray Ionization (ESI) coupled with High-Resolution Mass Spectrometry (HRMS) can determine the exact molecular formula by measuring the mass-to-charge ratio (m/z) with high precision. This is critical for confirming the identity of the compound and detecting potential impurities or byproducts with different molecular weights.

For procurement managers looking to buy 2-Hydroxy-6-methyl-5-nitropyridine, requesting comprehensive spectroscopic data from the supplier is standard practice. This data not only validates the product's identity and purity but also provides essential reference points for in-house analytical work. Understanding the typical spectroscopic signatures helps in ensuring that the material received consistently matches the required quality standards.

When sourcing from manufacturers, especially those located in China, partnering with a supplier that offers detailed analytical reports is key. We provide thorough analytical data for our products, including 2-Hydroxy-6-methyl-5-nitropyridine (CAS 28489-45-4), to support our clients’ quality control and R&D efforts. Ensuring the integrity of your chemical intermediates is our priority.

In summary, a suite of spectroscopic techniques, including NMR, IR, and MS, are indispensable tools for characterizing 2-Hydroxy-6-methyl-5-nitropyridine. These methods provide definitive proof of structure and purity, empowering chemists and formulators to proceed with confidence in their synthesis and product development endeavors.