Achieving effective anticoagulation with Ethylenediaminetetraacetic acid tripotassium salt (EDTA K3, CAS 65501-24-8) requires precise dosage and careful handling. As a manufacturer and supplier of this critical blood collection additive, we emphasize the importance of adhering to recommended guidelines to guarantee sample integrity and accurate diagnostic results.

The industry standard for vacuum blood collection typically recommends a dosage range of 1.5-2.2 mg of tripotassium EDTA per milliliter of blood. For specific applications, such as venous vacuum blood collection tubes, our company recommends 1.8-2.0 mg of Tripotassium EDTA per 1ml of blood to achieve optimal anticoagulation. Insufficient mixing or incorrect dosage can lead to issues such as platelet aggregation or microclot formation, compromising the quality of the blood sample. Proper mixing involves gently inverting the tube 9-10 times immediately after blood collection to ensure thorough dissolution and distribution of the anticoagulant.

Handling precautions are also vital. While EDTA K3 is stable at room temperature, it is hygroscopic, meaning it can absorb moisture from the air. Therefore, it should be stored in dry, sealed containers. In environments with temperatures exceeding 25°C, timely and sufficient mixing is especially critical to prevent localized coagulation. Furthermore, while EDTA K3 is an excellent anticoagulant for general hematological tests, it is not suitable for coagulation tests or platelet kinetic energy tests as it chelates calcium ions, which are essential for these assays. Understanding these nuances is key for laboratory professionals. As a reliable supplier, we provide high-purity EDTA Tripotassium Salt and are committed to offering guidance on its proper use. Sourcing from us ensures you receive a product that meets stringent quality standards, backed by our expertise.