The ubiquitin-proteasome system (UPS) is a fundamental cellular pathway responsible for protein homeostasis, involving the controlled degradation of proteins through ubiquitination. Deubiquitinating enzymes (DUBs) are critical regulators within this system, reversing the ubiquitination process. Ubiquitin C-terminal Hydrolase L3 (UCH-L3) is one such DUB, playing a key role in the maturation of ubiquitin precursors and the recycling of ubiquitin from protein substrates. Dysregulation of UCH-L3 has been linked to various diseases, making it an important target for research and potential therapeutic development.

To effectively study the specific functions and cellular roles of UCH-L3, researchers rely on specialized chemical inhibitors. 4,5,6,7-Tetrachloro-1H-indene-1,3(2H)-dione (TCID) is a prominent example of such a compound. TCID is recognized for its potent inhibitory activity against UCH-L3, with a reported IC50 of 0.6 µM. Furthermore, it exhibits significant selectivity, showing over 125 times greater selectivity for UCH-L3 compared to UCH-L1, another important DUB. This high selectivity ensures that research outcomes are more accurately attributed to the modulation of UCH-L3 activity, minimizing off-target effects. Researchers can confidently buy TCID from specialized suppliers to advance their studies.

As a cell-permeable inhibitor, TCID can effectively reach its target within living cells, allowing for in-depth analysis of UCH-L3's impact on cellular processes. Its applications span various research areas, including neurobiology, where it has been used to study the ubiquitination of transporters and its implications for neurological conditions. When looking to purchase TCID, it is crucial to source from a reputable TCID supplier that guarantees high purity and provides essential documentation such as Certificates of Analysis (CoA). Many leading chemical companies, particularly those based in China, offer TCID, providing researchers with access to quality materials at competitive prices.

For academic institutions and pharmaceutical companies, securing a reliable supply of high-quality TCID is essential for consistent research results and potential drug development efforts. Engaging with a well-established TCID manufacturer ensures that you receive a product that meets stringent quality standards. Exploring options for bulk purchases can also lead to cost efficiencies. By providing researchers with effective tools like TCID, the scientific community can gain deeper insights into the ubiquitin pathway and uncover new therapeutic strategies for diseases associated with UCH-L3 dysregulation.