At NINGBO INNO PHARMCHEM CO.,LTD., we are dedicated to advancing scientific discovery through high-quality chemical reagents. One such powerful tool is N-azidoacetylgalactosamine-tetraacylated, commonly known as Ac4GalNAz (CAS: 653600-56-7). This unnatural monosaccharide building block has become indispensable for researchers seeking to unravel the complexities of glycobiology.

Ac4GalNAz offers a sophisticated approach to metabolic labeling. Unlike traditional methods, Ac4GalNAz is cell-permeable and, once inside the cell, is processed to replace its natural counterpart, N-Acetylgalactosamine (GalNAc). This elegant incorporation leads to the creation of azide-functionalized glycoproteins within the cell. The beauty of this process lies in the azide moiety, which acts as a specific tag for subsequent detection.

The true power of Ac4GalNAz is unlocked through its compatibility with click chemistry. Researchers can utilize either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or copper-free click reactions to conjugate the azide-tagged glycoproteins with a variety of molecules. This includes fluorescent dyes for visualization under microscopy or biotin-alkyne for affinity purification and further analysis. This precise and efficient method significantly enhances our ability to study glycoprotein dynamics and functions.

The acetyl groups present in Ac4GalNAz are not merely for aesthetic purposes; they play a crucial role in enhancing the reagent's solubility in common organic solvents and improving its handling. This makes it easier for scientists to prepare and use Ac4GalNAz in their experimental setups, contributing to more reproducible and reliable results. As a key component for N-azidoacetylgalactosamine-tetraacylated metabolic labeling, Ac4GalNAz empowers researchers to achieve unprecedented insights.

By leveraging the capabilities of Ac4GalNAz click chemistry, scientists can delve deeper into understanding post-translational modifications, cell-cell interactions mediated by glycans, and the role of glycosylation in various biological processes and disease states. The ability to perform precise azide-labeled monosaccharide for glycoprotein detection opens new avenues for drug discovery and diagnostics.

NINGBO INNO PHARMCHEM CO.,LTD. is proud to supply Ac4GalNAz, a critical reagent for advancing metabolic glycoengineering tools and exploring the potential of cell-permeable unnatural sugars in modern life sciences. We are committed to providing high-quality intermediates that drive innovation.