The Role of BCIP p-Toluidine Salt in Modern Biochemical Assays
In the dynamic field of biochemistry, precise and reliable detection methods are paramount. Among the essential reagents facilitating these advancements is BCIP p-toluidine salt, scientifically known as 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (CAS 6578-06-9). This compound has carved a significant niche as a chromogenic substrate, particularly for the enzyme alkaline phosphatase.
The utility of BCIP p-toluidine salt stems from its ability to produce a visible color change upon enzymatic hydrolysis. When alkaline phosphatase cleaves the phosphate group, it releases an indoxyl intermediate. This intermediate, upon oxidation, forms an insoluble indigo precipitate, thereby visually indicating the presence and activity of the enzyme. This characteristic makes it invaluable in various analytical techniques, including Western blotting, immunohistochemistry, and in situ hybridization, where the detection of specific proteins or nucleic acid sequences is crucial.
As a key pharmaceutical intermediate, BCIP p-toluidine salt plays a role in the synthesis pathways of various drug compounds. Its consistent high purity, often exceeding 99% assay, is a testament to the rigorous manufacturing standards employed by leading Chinese suppliers. For researchers and manufacturers looking to buy BCIP p-toluidine salt, sourcing from a reputable Chinese manufacturer ensures not only competitive pricing but also a reliable supply chain, critical for uninterrupted research and production schedules. The availability of this compound at a wholesale price further democratizes access to high-quality biochemical reagents.
Furthermore, its application extends to agrochemical synthesis, where it can serve as an intermediate in the creation of specialized agricultural products. The versatility of BCIP p-toluidine salt as a chemical intermediate underscores its importance across multiple scientific and industrial sectors. Understanding the nuances of sourcing and utilizing this compound can significantly enhance experimental outcomes and product development.
For those seeking to procure this vital reagent, exploring options from established Chinese suppliers is a strategic move. These manufacturers often provide detailed specifications, certificates of analysis, and robust customer support, ensuring that you receive a product that meets stringent quality requirements for your specific needs, whether for chromogenic assay development or as a crucial intermediate.
The utility of BCIP p-toluidine salt stems from its ability to produce a visible color change upon enzymatic hydrolysis. When alkaline phosphatase cleaves the phosphate group, it releases an indoxyl intermediate. This intermediate, upon oxidation, forms an insoluble indigo precipitate, thereby visually indicating the presence and activity of the enzyme. This characteristic makes it invaluable in various analytical techniques, including Western blotting, immunohistochemistry, and in situ hybridization, where the detection of specific proteins or nucleic acid sequences is crucial.
As a key pharmaceutical intermediate, BCIP p-toluidine salt plays a role in the synthesis pathways of various drug compounds. Its consistent high purity, often exceeding 99% assay, is a testament to the rigorous manufacturing standards employed by leading Chinese suppliers. For researchers and manufacturers looking to buy BCIP p-toluidine salt, sourcing from a reputable Chinese manufacturer ensures not only competitive pricing but also a reliable supply chain, critical for uninterrupted research and production schedules. The availability of this compound at a wholesale price further democratizes access to high-quality biochemical reagents.
Furthermore, its application extends to agrochemical synthesis, where it can serve as an intermediate in the creation of specialized agricultural products. The versatility of BCIP p-toluidine salt as a chemical intermediate underscores its importance across multiple scientific and industrial sectors. Understanding the nuances of sourcing and utilizing this compound can significantly enhance experimental outcomes and product development.
For those seeking to procure this vital reagent, exploring options from established Chinese suppliers is a strategic move. These manufacturers often provide detailed specifications, certificates of analysis, and robust customer support, ensuring that you receive a product that meets stringent quality requirements for your specific needs, whether for chromogenic assay development or as a crucial intermediate.
Perspectives & Insights
Data Seeker X
“Furthermore, its application extends to agrochemical synthesis, where it can serve as an intermediate in the creation of specialized agricultural products.”
Chem Reader AI
“The versatility of BCIP p-toluidine salt as a chemical intermediate underscores its importance across multiple scientific and industrial sectors.”
Agile Vision 2025
“Understanding the nuances of sourcing and utilizing this compound can significantly enhance experimental outcomes and product development.”