Tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl), with CAS number 51805-45-9, is a compound celebrated in scientific circles for its exceptional properties as a reducing agent. Its unique chemical structure and performance characteristics make it a preferred choice for a multitude of laboratory applications, especially where the precise manipulation of disulfide bonds is crucial. Understanding the science behind TCEP-HCl can empower researchers to optimize their experiments and troubleshoot potential issues, making it easier to buy the right reagent from a reliable supplier.

The efficacy of TCEP-HCl as a reducing agent stems from its phosphine core, which readily donates electrons to break disulfide bonds (S-S) and form stable thiols (SH). Unlike traditional thiol-based reducing agents like DTT, TCEP-HCl is non-volatile, odorless, and significantly more stable in aqueous solutions, particularly at physiological pH. This stability ensures consistent reducing potential over extended periods, which is vital for long-term protein storage or complex multi-step reactions. Scientists often seek to purchase TCEP-HCl for its reliability in assays involving sensitive proteins and nucleic acids, such as in the development of new crown nucleic acid detection kits.

Furthermore, TCEP-HCl exhibits a unique advantage in its resistance to metal ions. Many biochemical processes utilize metal cofactors or affinity chromatography techniques that can deactivate thiol-based reducing agents. TCEP-HCl, however, remains effective even in the presence of moderate concentrations of metal ions, such as Ni2+ leached from affinity columns. This selectivity makes it an invaluable tool for researchers working with protein purification and analysis. The consistent quality and purity offered by leading manufacturers are essential for these precise scientific endeavors.

The application of TCEP-HCl as a pharmaceutical intermediate also highlights its scientific importance. Its controlled reactivity and stability make it suitable for incorporation into synthetic pathways where mild reduction is required. For companies involved in drug discovery and development, securing a consistent supply of high-purity TCEP-HCl is critical. Engaging with experienced chemical suppliers ensures not only product quality but also access to technical support and fair pricing. Inquiring about wholesale price for bulk quantities is a common practice for research institutions and manufacturing firms.

In summary, Tris(2-carboxyethyl)phosphine hydrochloride (CAS 51805-45-9) is a powerful and versatile chemical with profound scientific applications. Its superior reducing capabilities, stability, and selectivity make it a cornerstone reagent for researchers and developers alike. We, as a dedicated manufacturer, are proud to supply high-quality TCEP-HCl. We invite you to contact us for a quote and to discuss how our reliable product can advance your scientific endeavors.