Enzyme kinetics is a cornerstone of biochemistry, providing critical insights into how enzymes function, catalyze reactions, and interact with substrates and inhibitors. Understanding these dynamics is vital for fields ranging from drug development to metabolic research. At the heart of many enzyme kinetic studies lies the need for pure, reliable substrates. This article focuses on 2'-Deoxyuridine 5'-monophosphate disodium salt and its pivotal role in studying enzyme kinetics, particularly concerning thymidylate synthetase (TS).

Thymidylate synthetase (TS) is an enzyme that plays a crucial role in DNA synthesis by catalyzing the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). This reaction is essential for cell proliferation, making TS a significant target for cancer chemotherapy. To accurately study the kinetics of TS – including its reaction rates, substrate binding, and inhibition patterns – researchers require a precisely quantified amount of its substrate, dUMP.

2'-Deoxyuridine 5'-monophosphate disodium salt serves as an excellent reference substrate for these investigations. Its well-defined chemical structure and high purity, typically u226598.0% by HPLC, ensure that experimental results are accurate and reproducible. By employing this compound, scientists can determine key kinetic parameters such as Vmax (maximum reaction velocity) and Km (Michaelis constant), which are fundamental to understanding enzyme efficiency.

Moreover, this compound is also instrumental in assessing the efficacy of potential TS inhibitors. By measuring the reduction in dTMP production when inhibitors are present alongside the reference substrate, researchers can quantify the inhibitory potential of new drug candidates. This process is essential in the early stages of drug discovery and development, particularly for anticancer agents.

For laboratories engaged in detailed enzyme kinetic studies, sourcing high-quality biochemicals is critical. NINGBO INNO PHARMCHEM CO., LTD., a leading manufacturer in China, offers 2'-Deoxyuridine 5'-monophosphate disodium salt. Researchers looking to buy this essential reagent can trust the purity and consistency provided by NINGBO INNO PHARMCHEM CO., LTD. to ensure the validity of their kinetic data. The availability of such reliable materials from a trusted supplier expedites research progress.

In summary, 2'-Deoxyuridine 5'-monophosphate disodium salt is an indispensable tool for anyone studying enzyme kinetics, especially concerning the critical enzyme thymidylate synthetase. Its utility in both characterizing enzyme function and evaluating potential inhibitors makes it a valuable asset in biochemical research. NINGBO INNO PHARMCHEM CO., LTD. is proud to supply this high-purity compound, enabling scientists to achieve accurate and meaningful results in their pursuit of knowledge and therapeutic innovation.