Understanding the metabolic fate of chemical compounds is crucial, especially in pharmaceutical and agrochemical development, to assess efficacy, safety, and potential toxicological effects. 2-Fluoro-4-methylaniline (CAS: 452-80-2), a widely used intermediate, has been subjected to metabolic studies, primarily using in vitro systems like rat liver microsomes. NINGBO INNO PHARMCHEM CO.,LTD., a dedicated manufacturer and supplier, recognizes the importance of this knowledge for its clients. While we focus on providing high-quality chemical products, understanding the potential biotransformation pathways of our intermediates is part of our commitment to responsible chemical provision.

Key Metabolic Pathways Identified
Research into the metabolism of 2-Fluoro-4-methylaniline has revealed several primary pathways. One significant route involves the hydroxylation of the methyl group, leading to benzyl alcohol derivatives. These can be further oxidized to aldehydes. Another prominent pathway is N-hydroxylation of the amino group, forming hydroxylamines. Additionally, studies have identified the formation of secondary amine metabolites, such as halogenated N-(4'-aminobenzyl)-4-methylaniline, and even more complex derivatives like azoxy, azo, and hydrazo compounds.

Influence of Halogen Substituents on Metabolism
The presence and position of halogen substituents, such as the fluorine atom in 2-Fluoro-4-methylaniline, can significantly influence the rates and preferred routes of metabolism. Comparative studies with chlorinated and brominated analogues indicate that the halogen's electronic properties can shift metabolic activity between side-chain hydroxylation and N-hydroxylation. For instance, the rate of side-chain hydroxylation tends to be lower for 2-Fluoro-4-methylaniline compared to its chloro and bromo counterparts, while N-hydroxylation rates can be higher. This nuanced behavior underscores the importance of structural details in predicting metabolic profiles.

Reactive Metabolites and Toxicological Considerations
A critical aspect of xenobiotic metabolism is the potential formation of reactive intermediates that can bind to cellular macromolecules, leading to toxicity. While specific studies on 2-Fluoro-4-methylaniline's reactive metabolite formation are ongoing, research on related fluorinated anilines has highlighted the generation of quinoneimine intermediates. These electrophilic species can react with cellular nucleophiles, raising toxicological concerns. Understanding these pathways is vital for risk assessment and for designing safer chemical entities. It is important for users to handle this compound with appropriate safety precautions, as recommended by its Material Safety Data Sheet (MSDS).

Sourcing High-Purity Intermediates for Research
NINGBO INNO PHARMCHEM CO.,LTD. provides 2-Fluoro-4-methylaniline with a purity of ≥98%, ensuring that studies on its properties and transformations are conducted with reliable materials. Whether you are investigating its synthetic utility, its biological activity, or its metabolic fate, sourcing from a reputable manufacturer like us guarantees the quality needed for accurate research. We offer competitive prices and are a dependable supplier for the research community. We encourage you to buy with confidence from us.

Further Research and Safety Awareness
For detailed information on handling and safety protocols, always refer to the product's MSDS. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing chemical intermediates that support rigorous scientific investigation and industrial application, while emphasizing safe handling and awareness of potential metabolic pathways.