Understanding Ponceau S: Properties, Applications, and Sourcing
Ponceau S, a synthetic azo dye with the CAS number 6226-79-5, is a critical reagent in many biological and biochemical laboratories. Its primary utility lies in its function as a rapid, reversible stain for proteins. This characteristic makes it particularly valuable in the context of Western blotting, a widely used technique for identifying specific proteins in a sample. As a dedicated chemical manufacturer, we provide high-quality Ponceau S to support diverse research needs.
Chemically, Ponceau S is a disodium salt and a water-soluble dye. Its molecular structure allows it to interact with proteins through electrostatic forces and non-covalent bonding. Specifically, it binds to positively charged amino groups on proteins and also interacts with non-polar regions. This binding makes protein bands visible on membranes such as nitrocellulose, PVDF, and cellulose acetate. The dye's distinct red-brown color, when dissolved, allows for easy visual identification of protein loading and transfer efficiency. For those seeking to purchase Ponceau S, understanding these properties ensures optimal application.
The applications of Ponceau S extend beyond just Western blotting. It can be used as a general protein stain for membranes, offering a quick assessment of protein presence before more specific detection methods are employed. Its reversibility is a significant advantage, allowing for subsequent probing with antibodies without permanently altering the protein structure. This makes it an integral part of many protein analysis workflows. When considering where to buy Ponceau S, partnering with a reputable manufacturer in China can ensure both quality and cost-effectiveness, especially for bulk orders.
As a trusted supplier, we are committed to providing laboratory professionals with reliable access to essential biochemicals like Ponceau S. Our focus is on ensuring product consistency and purity, backed by efficient service and competitive pricing. Whether you are performing routine Western blots or developing new protein analysis techniques, sourcing your Ponceau S from a dependable manufacturer like us will ensure the success of your experiments. Contact us today to learn more or to get a quote for your bulk purchase requirements.
Perspectives & Insights
Chem Catalyst Pro
“For those seeking to purchase Ponceau S, understanding these properties ensures optimal application.”
Agile Thinker 7
“It can be used as a general protein stain for membranes, offering a quick assessment of protein presence before more specific detection methods are employed.”
Logic Spark 24
“Its reversibility is a significant advantage, allowing for subsequent probing with antibodies without permanently altering the protein structure.”