Fluorescein Sodium Salt, a derivative of fluorescein, is a compound celebrated for its intense fluorescence and remarkable versatility. Its chemical structure and properties make it a valuable reagent in numerous laboratory applications, ranging from analytical chemistry to materials science. Understanding its fundamental chemical characteristics is key to appreciating its broad utility.

Chemically, Fluorescein Sodium Salt is synthesized from resorcinol and phthalic anhydride. The resulting molecule, fluorescein, is then converted into its disodium salt form, which significantly enhances its solubility in water. This increased water solubility is a critical advantage, simplifying its use in aqueous solutions and enabling its application in a wider array of experimental setups compared to its less soluble parent compound. Its molecular formula is C20H10Na2O5, and it typically appears as an orange or orange-red powder.

The spectral properties of Fluorescein Sodium Salt are central to its functionality. It exhibits strong absorption of light in the blue region of the visible spectrum, typically with a peak absorption wavelength around 490 nm. Upon excitation, it emits light in the green region, with peak emission around 515 nm. This distinct excitation and emission profile is what makes it so effective as a fluorescent probe. The intensity of its fluorescence is also sensitive to pH, with optimal fluorescence occurring in alkaline conditions, a property that can be exploited in certain analytical applications.

In analytical chemistry, Fluorescein Sodium Salt is employed in various titrations as an indicator. Its color change and fluorescence can signal the endpoint of certain chemical reactions. It is also used in spectrofluorometry for quantitative analysis, where the intensity of its fluorescence can be correlated with the concentration of an analyte. Researchers also utilize it in studies involving membrane permeability and transport phenomena, leveraging its ability to cross certain barriers or bind to specific cellular components.

The stability and well-defined spectral characteristics of Fluorescein Sodium Salt make it a reliable laboratory reagent. Its predictable behavior under various conditions ensures consistency in experimental results. NINGBO INNO PHARMCHEM CO.,LTD. provides high-purity Fluorescein Sodium Salt, ensuring that scientists and chemists have access to a dependable reagent for their analytical and research needs. This commitment to quality supports innovation across many chemical and scientific disciplines.