Diethyl Pyrocarbonate (DEPC), identified by CAS number 1609-47-8, is a chemical compound with significant applications in biological research. Primarily recognized for its role in molecular biology, DEPC is extensively used to prepare DEPC-treated water, a critical component for ensuring RNase-free conditions essential for RNA isolation and subsequent analyses like cDNA synthesis and gene expression studies. Its ability to irreversibly inhibit RNase enzymes makes it a vital reagent for preserving the integrity of RNA samples, which are inherently susceptible to degradation.

Beyond its function in RNA preservation, DEPC also serves as a valuable reagent in protein chemistry. It can modify specific amino acid residues within proteins, most notably histidine and tyrosine. This modification capability is utilized in various biochemical studies, such as probing protein active sites, understanding enzyme mechanisms, and developing functional assays. Researchers aiming to buy DEPC for these purposes often seek high-purity grades to ensure the specificity and reliability of their experimental outcomes. As a manufacturer, we prioritize delivering DEPC that meets these exacting standards.

Handling Diethyl Pyrocarbonate requires careful consideration due to its reactive nature. While essential for research, it is important to follow proper laboratory safety protocols. This includes adequate ventilation, wearing appropriate personal protective equipment (PPE) such as gloves and eye protection, and storing the chemical in a cool, dry place away from direct sunlight. The successful application of DEPC, whether for creating RNase-free water or for protein modification, hinges on understanding its properties and handling it responsibly. Our company, as a dependable supplier in China, is committed to providing researchers with not only high-quality DEPC but also the necessary information to use it safely and effectively, thereby supporting advancements in biological sciences.