Benzotriazol-1-yloxytris(dimethylamino)phosphonium Hexafluorophosphate, widely known as BOP reagent, is a powerful and versatile coupling agent crucial in modern organic synthesis. With its Chemical Abstracts Service (CAS) number 56602-33-6, this compound plays an indispensable role, particularly in the formation of amide bonds, which is fundamental to peptide synthesis.

Chemically, BOP reagent is characterized by its molecular formula C12H22F6N6OP2 and a molecular weight of 442.287 g/mol. It typically presents as a white to off-white crystalline powder. Key physical properties include a melting point ranging from 136 to 140 °C. While it exhibits partial solubility and decomposes in cold water, it is readily soluble in common organic solvents such as methanol, acetone, and dichloromethane, which facilitates its application in various reaction environments. Stability is maintained under normal temperatures and pressures, though it is recognized as sensitive to both moisture and light, necessitating appropriate storage conditions, typically between 2-8°C.

The primary and most significant application of BOP reagent lies in peptide synthesis, where it efficiently mediates the formation of amide bonds between carboxylic acids and amines. A notable advantage of using BOP reagent in this context is its ability to circumvent undesirable side reactions, such as the dehydration of asparagine or glutamine residues, which can be problematic with other coupling methods. This specificity contributes to higher yields and purer products, making it a preferred choice for complex peptide constructions.

Beyond its central role in peptide chemistry, BOP reagent demonstrates remarkable versatility in other synthetic transformations. It has been effectively employed in the synthesis of esters from carboxylic acids and alcohols, offering a mild and efficient pathway for these valuable compounds. Furthermore, BOP reagent, when used in conjunction with sodium borohydride (NaBH4), serves as a chemoselective reagent for the reduction of carboxylic acids to primary alcohols, providing a valuable tool for chemists seeking to modify carboxylic acid functionalities without affecting other sensitive groups in the molecule.

It is important to note a safety consideration associated with the use of BOP reagent: the production of hexamethylphosphoramide (HMPA) as a stoichiometric by-product. HMPA is recognized as a carcinogenic compound, which has led to some discouragement of BOP reagent's use in large-scale industrial applications. However, for small-scale laboratory syntheses, this concern is generally mitigated, and its efficacy often outweighs the disadvantage, especially given proper handling and ventilation protocols.

At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to being a reliable manufacturer and supplier of high-purity Benzotriazol-1-yloxytris(dimethylamino)phosphonium Hexafluorophosphate (CAS: 56602-33-6). Our dedication to quality control ensures that every batch meets rigorous purity standards, typically at 95% min., providing our clients with a consistent and effective product for their research and development needs. We understand the critical importance of reliable chemical reagents in advancing scientific discovery and pharmaceutical development.

For those looking to buy or purchase this essential peptide coupling reagent, NINGBO INNO PHARMCHEM CO.,LTD. offers competitive price options and a streamlined ordering process. We maintain a robust supply chain to ensure prompt delivery and provide technical support to assist our customers. Our focus is on fostering long-term partnerships by delivering superior products and exceptional service. We invite you to contact us for inquiries regarding bulk orders, specific packaging requirements, or any further technical specifications.