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The selection of an appropriate chromogenic substrate is a critical decision for researchers and assay developers aiming for optimal performance in biochemical assays. Among the various options available, N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt (TMBZ) stands out due to its advantageous properties. Ningbo INNO Pharmchem Co., Ltd. outlines why TMBZ is often the preferred choice for sensitive detection systems.
TMBZ's primary advantage lies in its high sensitivity and its ability to produce a clear, quantifiable color change when oxidized by peroxidase enzymes. Unlike some older chromogenic substrates, TMBZ is water-soluble, which greatly simplifies its preparation and use in aqueous buffer systems common in biological assays. This characteristic makes it an attractive option for those searching for 'water-soluble chromogenic substrate benefits' or 'TMBZ ease of use'. The substrate reacts with hydrogen peroxide catalyzed by peroxidase, leading to a color development that is easily measured spectrophotometrically. The reaction can be stopped with acid, typically yielding a yellow color with strong absorbance at 450 nm.
Furthermore, TMBZ is known for its excellent stability and a relatively low background signal compared to other substrates. This combination is crucial for assays where detecting low levels of analytes is essential, such as in early-stage disease diagnostics or quantitative protein analysis. When considering 'TMBZ stability for assays', users are seeking reliable reagents that provide consistent results over time and across different experimental runs. This is a key consideration for laboratory reagent manufacturing, ensuring product quality and reproducibility.
The versatility of TMBZ also extends to its use in various immunoassay formats, including ELISA and immunohistochemistry. Its reliable performance and ease of integration into established protocols make it a valuable tool for both routine diagnostics and cutting-edge research. Ningbo INNO Pharmchem Co., Ltd. is dedicated to supplying high-quality TMBZ, ensuring that scientists have access to a dependable chromogenic substrate that enhances the accuracy and efficiency of their critical biochemical assays.
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1. 20 years of R&D, manufacturing and sales experience, serving customers in 60 countries and regions around the world;
2. Own R&D laboratory, pilot platform and large-scale production workshop, which can meet the audit requirements of global customers;
3. We can satisfy customers' perfect transition from small scale lab requirements (gram level) to commercialization requirements (hundred tons level).
A: We don't have Minimum Order Quantity, exact quantity should be provided before quotation for us to calculate the exact cost.
A: We don't provide free samples due to lots of request and expensive international courier's cost, we can deduct the sample charge after commercial order placed.
A: Our payment terms: Small or sample order: T/T IN ADVANCE. Commercial order: First order should be by T/T IN ADVANCE or L/C at sight, and following orders T/T 30~90days is acceptable subject to approval of credit application.
