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The Enzyme-Linked Immunosorbent Assay (ELISA) is a cornerstone technique in biological and medical laboratories, widely used for detecting and quantifying antibodies, antigens, proteins, and hormones. The performance of an ELISA is heavily influenced by its critical components, among which the chromogenic substrate plays a pivotal role. Ningbo INNO Pharmchem Co., Ltd. highlights the importance of N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt as a superior chromogenic substrate for enhancing ELISA accuracy.
As a water-soluble compound, N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt offers convenience in preparation and application within ELISA protocols. Its primary function is to act as a chromogenic indicator for the activity of peroxidase enzymes, which are typically conjugated to detection antibodies. In the presence of hydrogen peroxide, the substrate is oxidized, yielding a colored product. The intensity of this color is directly proportional to the amount of enzyme present, and thus, the target analyte in the sample. The transition from a colorless solution to a blue hue, which later turns yellow upon acidification, provides a clear and quantifiable signal.
Researchers often search for 'TMBZ supplier' or 'where to buy TMBZ' when aiming to improve their assay sensitivity and reduce background noise. The low background signal characteristic of this substrate is a significant advantage, allowing for the detection of even very low concentrations of the target molecule. This enhanced sensitivity is crucial for applications requiring early detection of diseases or monitoring minute changes in biomarker levels. This directly addresses the need for reliable enzyme assay substrates in scientific research.
The consistent quality and stability of N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine sodium salt ensure reproducible results, a critical factor for laboratories performing diagnostic tests or conducting rigorous scientific studies. Ningbo INNO Pharmchem Co., Ltd. is committed to providing such high-quality reagents, understanding that the reliability of diagnostic tests hinges on the performance of each component. By choosing robust chromogenic substrates, labs can confidently achieve the precision needed for effective biological analysis and ensure the accuracy of their ELISA results.
Manufacturing Facilities
Professional Export Experience
to Global Customers
1. 20 years of R&D, manufacturing and sales experience, serving customers in 60 countries and regions around the world;
2. Own R&D laboratory, pilot platform and large-scale production workshop, which can meet the audit requirements of global customers;
3. We can satisfy customers' perfect transition from small scale lab requirements (gram level) to commercialization requirements (hundred tons level).
A: We don't have Minimum Order Quantity, exact quantity should be provided before quotation for us to calculate the exact cost.
A: We don't provide free samples due to lots of request and expensive international courier's cost, we can deduct the sample charge after commercial order placed.
A: Our payment terms: Small or sample order: T/T IN ADVANCE. Commercial order: First order should be by T/T IN ADVANCE or L/C at sight, and following orders T/T 30~90days is acceptable subject to approval of credit application.
