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Understanding the chemical foundation of research reagents is key to unlocking their full potential. NINGBO INNO PHARMCHEM CO.,LTD. provides insight into Fast Blue B Salt (CAS 14263-94-6), a compound whose chemical properties make it highly effective in various biological research applications, particularly in histochemistry and biochemical assays. Its structure and reactivity are central to its function as a precise detection tool.
Chemically, Fast Blue B Salt is a diazo dye, specifically an organic chloride salt derived from 3,3'-dimethoxybiphenyl-4,4'-bis(diazonium) and zinc chloride. Its molecular formula, C14H12N4O2Cl2·ZnCl2, reflects its complex composition. The presence of diazonium groups is crucial for its reactivity, allowing it to couple with substrates or undergo reactions that produce a colored product. This characteristic is exploited in its primary function as a chromogenic substrate for enzyme detection.
When used in conjunction with enzymes like alkaline phosphatase, Fast Blue B Salt undergoes enzymatic cleavage. This process releases the diazo component, which then precipitates to form a vibrant blue color at the site of enzymatic activity. This direct visual cue is invaluable for researchers seeking to pinpoint the location and quantify the levels of specific enzymes within biological samples. The ability to buy Fast Blue B Salt of high purity, as offered by NINGBO INNO PHARMCHEM CO.,LTD., ensures the reliability of these colorimetric reactions.
Beyond enzyme detection, Fast Blue B Salt has found applications in demonstrating esterase activity and staining enterochromaffin cells in histological studies. Its ability to react with free radical oxidative damage also points to its utility in research investigating cellular stress and pathology. These diverse applications highlight the compound's versatility and its importance in advancing our understanding of biological processes.
For researchers focusing on precise analytical techniques, the physical properties of Fast Blue B Salt are also noteworthy. It typically appears as a yellow-green to dark brown powder and is soluble in water, forming a clear solution at specific concentrations. Proper storage, usually at 2-8°C, is recommended to maintain its stability and efficacy. NINGBO INNO PHARMCHEM CO.,LTD. ensures that these crucial properties are maintained in the products they supply, supporting consistent research outcomes.
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1. 20 years of R&D, manufacturing and sales experience, serving customers in 60 countries and regions around the world;
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3. We can satisfy customers' perfect transition from small scale lab requirements (gram level) to commercialization requirements (hundred tons level).
A: We don't have Minimum Order Quantity, exact quantity should be provided before quotation for us to calculate the exact cost.
A: We don't provide free samples due to lots of request and expensive international courier's cost, we can deduct the sample charge after commercial order placed.
A: Our payment terms: Small or sample order: T/T IN ADVANCE. Commercial order: First order should be by T/T IN ADVANCE or L/C at sight, and following orders T/T 30~90days is acceptable subject to approval of credit application.
