7-Amino-4-Methylcoumarin (AMC) is a prominent chemical compound recognized for its significant utility in various scientific disciplines, particularly in biochemistry, molecular biology, and analytical chemistry. This high-purity substance, typically presenting as a white powder, boasts a CAS number of 26093-31-2 and a molecular formula of C10H9NO2. Its purity is a critical factor for its effectiveness in sensitive applications, often exceeding 99%.

The primary value of 7-Amino-4-Methylcoumarin lies in its fluorescent properties. When excited, AMC emits blue light, making it an excellent fluorophore. This characteristic leads to its diverse range of applications. One of the most significant uses is as a fluorescent labeling reagent, particularly for enzymatic trace determination. AMC is widely employed to create fluorogenic substrates for various enzymes, such as proteases and peptidases. By conjugating AMC to a peptide or other molecule, the cleavage of the substrate by the enzyme releases the free, highly fluorescent AMC molecule, allowing for sensitive detection and quantification of enzyme activity. This makes AMC substrates invaluable tools for enzyme assays, high-throughput screening of enzyme inhibitors, and kinetic studies.

Beyond enzymatic applications, 7-Amino-4-Methylcoumarin serves as a versatile tool in analytical techniques. It can be used as a laser dye, contributing to its application in specialized optical systems. In the field of chromatography and mass spectrometry, AMC and its derivatives function as matrices or co-matrices for the analysis of complex biomolecules. For instance, it is utilized for the analysis of sulfated disaccharides and other complex oligosaccharides, providing enhanced detection sensitivity.

Furthermore, research indicates potential applications of 7-Amino-4-Methylcoumarin in biological studies, including investigations into its effects on cell morphology. Preliminary studies have also explored its potential as an antibacterial agent against certain resistant strains. Its role as a fluorescence reference standard is crucial for calibrating instruments and ensuring the reliability of fluorescence-based measurements in numerous research settings.

The demand for high-quality 7-Amino-4-Methylcoumarin powder stems directly from its critical roles in research, diagnostics, and specialized industrial applications. Ensuring a reliable supply of this compound is essential for laboratories and manufacturers. Researchers and companies looking to buy high purity 7-Amino-4-Methylcoumarin powder should source from a reputable supplier or manufacturer that adheres to strict quality control standards. The price of AMC can vary depending on purity, quantity, and supplier, making it important to obtain detailed quotations for specific requirements. When considering a purchase, factors such as product specification, packaging options (e.g., 1kg bags, 25kg drums), and transit methods are key considerations for efficient procurement.