NINGBO INNO PHARMCHEM CO.,LTD. is proud to present its premium offering of HEPES, a critically important biological buffer widely utilized across numerous scientific and industrial disciplines. As a leading manufacturer and supplier of high-quality chemical raw materials, we understand the stringent requirements for purity and consistency, particularly in sensitive applications like cell culture and biological research.

HEPES, chemically known as 4-hydroxyethyl piperazineethanesulfonic acid, is a zwitterionic buffer with the molecular formula C8H18N2O4S and a molecular weight of 238.305. Its CAS number is 7365-45-9. It typically presents as a white crystalline powder, highly soluble in water. The primary advantage of HEPES is its ability to maintain a stable pH within the range of 6.8 to 8.2, which is physiologically relevant for many biological systems. This stability is crucial because fluctuations in pH can significantly impact enzyme activity, protein structure, and cellular processes. Unlike some other buffers, HEPES does not complex with metal ions, further enhancing its suitability for biological applications where metal ions are present or required.

One of the most prominent uses of high-purity HEPES buffer is in cell culture media. Maintaining a stable pH environment is paramount for the healthy growth and survival of cells in vitro. While bicarbonate buffering systems are common, they rely on a controlled CO2 atmosphere, which is not always feasible. HEPES provides an alternative or supplementary buffering capacity independent of CO2 levels, making it ideal for open-dish cultures, transport of cells or tissues, or experiments conducted outside of CO2 incubators. A typical concentration of 20mmol/L in culture medium provides robust buffering capacity, ensuring the culture remains viable and experimental results are reliable. The high purity, often exceeding 99%, is non-negotiable for cell culture applications, as even trace impurities can be toxic to delicate cells.

Beyond cell culture, HEPES has found significant application in the cosmetics industry. Its buffering properties are utilized to stabilize the pH of cosmetic formulations, ensuring the efficacy and longevity of active ingredients. Many cosmetic ingredients, including microbial fermentation extracts and certain enzymes, are pH-sensitive. By maintaining an optimal pH, HEPES helps preserve their activity and the overall stability of the product. Furthermore, research suggests that HEPES can play a role in skin care formulations by assisting in keratin softening and promoting skin cell metabolism. This can contribute to improved skin texture, enhanced absorption of other beneficial ingredients, and potentially stronger skin barrier function. Its use in cosmetics underscores its non-toxic nature and compatibility with biological systems.

In molecular biology and biochemistry, HEPES is also a valuable reagent. It is used in various diagnostic kits, including those for DNA/RNA extraction and PCR (Polymerase Chain Reaction), where precise pH control is necessary for optimal enzyme activity and reaction efficiency. Its compatibility with enzymes like t4 RNA ligase makes it useful in nucleic acid manipulation protocols, such as RNA 3'-terminal marking. The stable and inert nature of HEPES ensures it does not interfere with these sensitive biochemical reactions.

At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to providing HEPES of the highest purity and quality. Our manufacturing processes adhere to strict quality control standards to ensure that every batch meets or exceeds customer expectations, particularly the requirement for purity over 99%. The product is supplied as a pure white crystal powder, with good water solubility and excellent thermal stability at room temperature, making it easy to handle and store under standard conditions (avoiding light and moisture). The stable molecular structure further guarantees its reliability over time.

For customers looking to buy or purchase high-quality HEPES, whether for laboratory research, pharmaceutical production, cosmetic formulation, or industrial applications, NINGBO INNO PHARMCHEM CO.,LTD. offers competitive pricing and flexible packaging options. We understand that quantities required can vary, from smaller laboratory needs to bulk industrial demands. Standard packaging includes options like 500g bottles or 25kg buckets, with possibilities for customized packaging upon request. Our goal is to be your reliable supplier for all your HEPES needs, providing a consistent and dependable source of this essential raw material.

Obtaining a clear understanding of the price structure and delivery options is straightforward. Interested parties are encouraged to contact our sales team for detailed quotations and information regarding minimum order quantities and lead times. As a dedicated manufacturer and supplier, we strive to offer excellent value without compromising on product quality. Our established supply chain ensures timely delivery to locations worldwide, facilitating your procurement process.

In conclusion, HEPES is an indispensable buffer across the life sciences and chemical industries due to its superior pH stability, high purity, and compatibility with biological systems. NINGBO INNO PHARMCHEM CO.,LTD. is your trusted partner for sourcing premium HEPES powder, offering the quality assurance and supply chain reliability you need to succeed in your research or manufacturing endeavors. We welcome inquiries for detailed product specifications, sample requests, and pricing information. Let us be your reliable supplier for high-purity HEPES.