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Understanding FITC Isomer I for Flow Cytometry & Immunoassays

Fluorescein Isothiocyanate Isomer I (FITC), CAS 3326-32-7, is a foundational fluorophore widely employed in cutting-edge biological and medical research. Its primary utility lies in its capacity to act as a fluorescent tag, enabling the visualization and quantification of biomolecules within complex biological systems. For scientists involved in flow cytometry and immunoassay development, understanding the nuances of FITC Isomer I and its sourcing is essential.

In flow cytometry, FITC-conjugated antibodies are instrumental in identifying and sorting cells based on the expression of specific surface or intracellular markers. The bright green emission of FITC, with an excitation peak around 492 nm and an emission peak around 518 nm, makes it compatible with common laser excitation sources, such as the 488 nm argon laser. This compatibility allows for clear detection of labeled cells, distinguishing them from unlabeled populations. When researchers seek to buy FITC protein labeling agents for these applications, they look for consistency and purity, qualities that NINGBO INNO PHARMCHEM CO.,LTD. consistently delivers.

Immunoassays, including ELISA and Western blotting, also heavily rely on the precise labeling capabilities of FITC Isomer I. By conjugating FITC to antibodies or antigens, researchers can detect and quantify the presence of specific analytes. The stable thiourea linkage formed between FITC and amine groups on proteins ensures the integrity of the label throughout the assay, leading to reliable and reproducible results. As a leading manufacturer and supplier of CAS 3326-32-7 in China, we provide researchers with the high-quality FITC necessary for sensitive and specific immunoassays.

The selection of a reliable supplier for biochemical reagents like Fluorescein Isothiocyanate Isomer I is critical for research integrity. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing scientists globally with premium reagents. We offer competitive pricing and robust support for those looking to purchase our FITC products. Our efficient supply chain from China ensures timely delivery, supporting the fast-paced demands of scientific discovery. Engage with us to secure your supply of this vital fluorescent labeling reagent and enhance your research outcomes.

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  • A: We don't have Minimum Order Quantity, exact quantity should be provided before quotation for us to calculate the exact cost.

  • A: We don't provide free samples due to lots of request and expensive international courier's cost, we can deduct the sample charge after commercial order placed.

  • A: Our payment terms: Small or sample order: T/T IN ADVANCE. Commercial order: First order should be by T/T IN ADVANCE or L/C at sight, and following orders T/T 30~90days is acceptable subject to approval of credit application.

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