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Phosphatase enzymes play crucial roles in numerous biological processes, and their accurate measurement is fundamental to biochemical research and clinical diagnostics. Disodium 4-Nitrophenyl Phosphate (PNPP) has emerged as a cornerstone reagent for detecting and quantifying the activity of these enzymes, particularly alkaline phosphatase (AP). As a leading chemical supplier, NINGBO INNO PHARMCHEM CO.,LTD. offers high-quality PNPP (CAS 4264-83-9), understanding its significance in various assay formats.
The mechanism behind PNPP's utility is straightforward yet powerful. When PNPP is incubated with a sample containing alkaline phosphatase activity, the enzyme catalyzes the hydrolysis of the phosphate ester bond. This reaction releases a molecule of p-nitrophenol, which is intensely yellow in alkaline conditions. The rate at which this yellow color develops is directly proportional to the alkaline phosphatase activity present in the sample. This chromogenic property allows for precise quantitative analysis using a spectrophotometer, typically measuring absorbance at around 405-410 nm.
This principle is widely applied in various fields, including immunoassays like ELISA, where AP is often used as a label. It's also critical in academic research for studying enzyme kinetics, characterizing enzyme inhibitors, and monitoring enzyme activity in different biological contexts. For professionals in these fields, sourcing a reliable and high-purity Disodium 4-Nitrophenyl Phosphate is essential to ensure assay reproducibility and accuracy. NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated manufacturer and supplier from China, is committed to meeting these stringent requirements.
We understand that purchasing bulk chemicals requires confidence in the supplier. Our goal is to provide cost-effective solutions without compromising on quality. If you are a procurement manager looking to buy PNPP, we offer competitive pricing and ensure a stable supply. We invite you to contact us for a detailed quote and to obtain a sample of our Disodium 4-Nitrophenyl Phosphate. Partnering with NINGBO INNO PHARMCHEM CO.,LTD. means securing a dependable source for a critical component of your phosphatase assays, backed by our commitment to excellence and customer satisfaction.
Manufacturing Facilities
Professional Export Experience
to Global Customers
1. 20 years of R&D, manufacturing and sales experience, serving customers in 60 countries and regions around the world;
2. Own R&D laboratory, pilot platform and large-scale production workshop, which can meet the audit requirements of global customers;
3. We can satisfy customers' perfect transition from small scale lab requirements (gram level) to commercialization requirements (hundred tons level).
A: We don't have Minimum Order Quantity, exact quantity should be provided before quotation for us to calculate the exact cost.
A: We don't provide free samples due to lots of request and expensive international courier's cost, we can deduct the sample charge after commercial order placed.
A: Our payment terms: Small or sample order: T/T IN ADVANCE. Commercial order: First order should be by T/T IN ADVANCE or L/C at sight, and following orders T/T 30~90days is acceptable subject to approval of credit application.
