NINGBO INNO PHARMCHEM CO.,LTD. understands the critical need for efficient and reliable laboratory techniques. N,N'-Bis(Acryloyl)Cystamine (CAS: 60984-57-8), often abbreviated as BAC, is a key reagent that significantly improves the efficiency of DNA purification processes through its application in polyacrylamide gel electrophoresis.

The core utility of BAC in this context stems from its function as a reversible cross-linking agent for polyacrylamide gels. Traditional methods of separating and isolating DNA can be laborious. By incorporating BAC into the gel matrix, researchers create a system where the cross-links, formed by the disulfide bond, can be intentionally broken. This is achieved by applying a suitable reducing agent, such as dithiothreitol (DTT) in a buffered solution. This targeted cleavage of the gel network allows for the gentle and effective release of the separated DNA molecules.

The advantage of using BAC for DNA purification is evident in the simplified workflow and the integrity of the isolated DNA. When a gel slice containing the target DNA is treated with a solution like 100 mM DTT in TBE at pH 8.3, the gel dissolves efficiently, typically within 30 minutes for a standard gel slice. This ease of dissolution minimizes physical stress on the DNA, preserving its structure and preventing degradation. The optimal pH of 8.3 further ensures rapid and complete gel dissolution, making the entire process more streamlined and effective.

For researchers focused on precise molecular biology applications, the reliability of reagents like N,N'-Bis(Acryloyl)Cystamine is paramount. Its consistent performance in creating cleavable gel networks ensures reproducible results. This chemical intermediate is therefore not just a component, but an enabler of accurate and high-yield DNA isolation, supporting advancements in genetic research, diagnostics, and biotechnology.

At NINGBO INNO PHARMCHEM CO.,LTD., we pride ourselves on providing high-purity chemical intermediates essential for demanding laboratory procedures. Our commitment to quality ensures that when you use N,N'-Bis(Acryloyl)Cystamine, you are employing a reagent that meets stringent standards, facilitating your research in DNA purification and other critical applications. The accessibility of such key reagents through reliable suppliers like us is vital for scientific progress.

In essence, the strategic use of N,N'-Bis(Acryloyl)Cystamine in gel-based separations offers a tangible improvement in the efficiency and effectiveness of DNA purification, making it a cornerstone reagent for molecular biologists worldwide.