The Anthrone Test, a cornerstone method for carbohydrate quantification, relies on the chemical reagent Anthrone (CAS 90-44-8). This test is widely recognized for its accuracy and sensitivity in determining total carbohydrate content in biological and industrial samples. The underlying principle involves a specific colorimetric reaction that occurs when Anthrone interacts with carbohydrates in the presence of a strong acid, typically sulfuric acid.

The reaction mechanism is multifaceted. Initially, the strong acid dehydrates the carbohydrate molecules to form furfural or hydroxymethylfurfural derivatives. These reactive intermediates then undergo condensation reactions with Anthrone, leading to the formation of intensely colored products. The resulting color, often a deep green or blue-green, is measured using a spectrophotometer at a specific wavelength. The intensity of the color is directly proportional to the concentration of carbohydrates in the sample, allowing for precise quantitative analysis. This reliable approach makes purchasing Anthrone online a common requirement for research laboratories.

The Anthrone-Sulfuric Acid Test is particularly valuable because it can quantify a broad range of carbohydrates, including monosaccharides, disaccharides, and polysaccharides. This makes it an excellent tool for measuring total reducing sugars. Researchers often buy Anthrone from NINGBO INNO PHARMCHEM CO.,LTD. to ensure they are using a high-purity reagent, which is crucial for obtaining accurate and reproducible analytical results. The availability of Anthrone at a reasonable price further supports its extensive use in scientific studies.

The applications of the Anthrone Test extend to various fields, including the analysis of glycogen in biological tissues, the assessment of carbohydrate content in plant extracts, and quality control in the food and beverage industry. Understanding the properties of Anthrone is essential for optimizing the assay conditions and achieving the best possible results. The continuous demand for accurate carbohydrate measurement ensures that Anthrone remains a vital component in the analytical chemist's toolkit.