At NINGBO INNO PHARMCHEM CO.,LTD., we understand the importance of robust and versatile chemical tools for cutting-edge research. Our N-azidoacetylgalactosamine-tetraacylated (Ac4GalNAz, CAS: 653600-56-7) is a prime example of a reagent that, when combined with the power of click chemistry, offers unparalleled capabilities in glycobiology.

Click chemistry, a set of highly efficient and selective reactions, has revolutionized bioconjugation and molecular labeling. Ac4GalNAz, with its strategically placed azide moiety, is perfectly suited to participate in these reactions. This allows for the facile attachment of reporter molecules, such as fluorescent dyes or affinity tags, to glycoproteins that have been metabolically labeled with Ac4GalNAz.

The process begins with the introduction of Ac4GalNAz into living cells. As a cell-permeable unnatural sugar, it is efficiently taken up and incorporated into cellular glycans. The resulting azide-functionalized glycans are then ready for the click reaction. Whether employing the well-established copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or the metal-free strain-promoted azide-alkyne cycloaddition (SPAAC), the result is a stable and specific linkage.

This synergistic approach is invaluable for glycoprotein detection and analysis. For instance, researchers can label the azide-modified glycoproteins with a fluorescent alkyne to visualize their cellular localization and trafficking using fluorescence microscopy. Alternatively, biotin-alkyne can be used to capture and purify these modified glycoproteins for subsequent mass spectrometry analysis, providing detailed information about their structure and modifications.

The reliability and high yields associated with Ac4GalNAz click chemistry make it a preferred method for many applications in glycoscience. The demand for precise N-azidoacetylgalactosamine-tetraacylated metabolic labeling continues to grow as scientists explore the intricate roles of glycosylation in health and disease.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to supporting this scientific progress by providing Ac4GalNAz as a high-purity pharmaceutical intermediate. We believe that by offering these advanced metabolic glycoengineering tools, we can help accelerate discoveries in the field.