In the continuous pursuit of understanding and enhancing health and longevity, the role of antioxidants has come under intense scrutiny. Antioxidants are compounds that protect our cells from damage caused by free radicals, which are unstable molecules implicated in aging and various diseases. To accurately measure the capacity of a substance to combat these harmful radicals, scientists rely on robust and reproducible analytical methods. Among these, the DPPH assay stands out as a widely adopted and effective technique, largely due to the unique properties of the DPPH molecule itself.

DPPH, or 2,2-Diphenyl-1-picrylhydrazyl (CAS 1898-66-4), is celebrated for its stability as a free radical. This stability is key to its function as a benchmark in antioxidant assays. The chemical structure of DPPH allows it to readily accept an electron or hydrogen radical. When DPPH, which is a deep violet color in solution, encounters an antioxidant capable of donating a hydrogen atom, it is converted into its reduced, non-radical form, DPPH-H. This transformation results in a loss of the violet color, which can be precisely measured using a spectrophotometer at a wavelength of approximately 517 nm. The extent of this color change is directly proportional to the concentration of the antioxidant present, providing a quantitative measure of its radical-scavenging activity.

The widespread use of DPPH in research underscores its importance as a reliable DPPH free radical scavenger. Laboratories across the globe utilize DPPH for screening new botanical extracts, synthesized compounds, and food ingredients for their antioxidant properties. This application is crucial for sectors ranging from pharmaceuticals and nutraceuticals to cosmetics and food preservation. The ease of execution and the clear colorimetric readout make the DPPH assay an accessible yet powerful tool for researchers aiming to quantify antioxidant power. Understanding the DPPH CAS 1898-66-4 uses is essential for anyone in the field of redox biology or natural product chemistry.

Furthermore, the compound's role as an electron paramagnetic resonance (EPR) signal standard highlights its versatility. Researchers involved in EPR spectroscopy can calibrate their instruments using DPPH, ensuring the accuracy of their free radical detection and characterization studies. This makes DPPH not just an assay reagent but also a fundamental standard in physical chemistry research. The consistent performance of DPPH as a hydrogen radical scavenger compound makes it an indispensable reagent for quality assurance and discovery research.

At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to supplying researchers with high-purity DPPH, enabling accurate and impactful scientific discoveries. Our dedication to quality ensures that each batch meets stringent standards, supporting your efforts in quantifying antioxidant power and advancing scientific understanding.