In the realm of biochemical research, the ability to accurately visualize and quantify proteins after separation by electrophoresis is fundamental. Several staining reagents are available, each with its own strengths and weaknesses. Among these, Acid Black 1 (CAS 1064-48-8) stands out for its specific applications and sensitivity, making it a vital component for many laboratories. Understanding its comparative advantages is crucial for researchers seeking to buy Acid Black 1 for their protein analysis needs.

One of the most widely used protein stains is Coomassie Brilliant Blue (CBB) R250. CBB R250 is known for its good sensitivity and relatively fast staining protocol. However, Acid Black 1, also known as Amido Black 10B, offers a distinct advantage in certain scenarios. Its detection sensitivity is approximately 20% of that of Coomassie Brilliant Blue R250. This means that Acid Black 1 can detect proteins at lower concentrations, making it an excellent choice for samples with low protein abundance or when subtle expression differences need to be identified. This increased sensitivity is a significant factor for researchers looking to purchase Acid Black 1.

The mechanism of staining also differs. Acid Black 1, being an acid dye, binds to the basic amino acid residues of proteins (like arginine, lysine, and histidine) through ionic interactions. This binding is typically enhanced by an acidic destaining solution, which helps remove unbound dye and background staining. This specificity allows for clear visualization of protein bands against a clean background, a crucial aspect for accurate analysis and documentation.

For researchers working with gels or membranes, the protocol for using Acid Black 1 is straightforward. Typically, proteins are first fixed on the gel or membrane using an acetic acid solution. The stained sample is then immersed in a solution of 0.1% Acid Black 1 in 7% (v/v) acetic acid for at least 2 hours. Following staining, destaining is performed with a similar acetic acid solution until the background is clear. This process yields sharp, distinct bands that are easily visible.

When sourcing Acid Black 1, the quality of the dye directly impacts its performance. A high-purity product from a reliable manufacturer ensures consistent and reproducible staining results. Impurities can lead to a diffuse background or artifactual bands, potentially confusing experimental interpretation. Therefore, procurement managers should prioritize suppliers who can guarantee the purity and consistency of their Acid Black 1.

NINGBO INNO PHARMCHEM CO.,LTD. serves as a dependable supplier of high-quality Acid Black 1, catering to the needs of research laboratories. We understand the importance of sensitive and reliable staining for scientific discovery. By offering competitive Acid Black 1 price points and ensuring consistent product quality, we aim to support researchers in their endeavors. If you are seeking to buy Acid Black 1 for your protein electrophoresis needs, consider us for a product that delivers on sensitivity and reproducibility from a trusted manufacturer in China.