Acid Black 1 (CAS 1064-48-8), a prominent member of the disazo dye family, possesses a unique chemical structure that dictates its valuable properties. Understanding these characteristics is key for researchers and industrial chemists to fully leverage its capabilities. As a dedicated manufacturer, we at NINGBO INNO PHARMCHEM CO.,LTD. have a deep understanding of its synthesis and properties, ensuring we provide a high-quality product to those looking to buy Acid Black 1.

Chemically, Acid Black 1 is identified as disodium; 4-amino-5-hydroxy-3-[(E)-(4-nitrophenyl)diazenyl]-6-[(E)-phenyldiazenyl]naphthalene-2,7-disulfonate. Its molecular formula is C22H14N6Na2O9S2, with a molecular weight of 616.49 g/mol. The presence of two azo (-N=N-) groups and two sulfonate (-SO3-) groups, along with amino (-NH2) and hydroxyl (-OH) functionalities, contributes to its dyeing capabilities and water solubility. The sulfonate groups, in particular, render it an acid dye, allowing it to form ionic bonds with positively charged sites on protein fibers and polyamide materials, ensuring strong adhesion and wash fastness.

The synthesis of Acid Black 1 typically involves a multi-step process of diazotization and coupling. First, p-nitroaniline is diazotized in acidic conditions. This diazonium salt is then coupled with 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid. Subsequently, aniline is diazotized and coupled under alkaline conditions to form the final disazo structure. This intricate synthesis route, when carried out with precision by a skilled manufacturer, yields a product with high purity and the desired chromophoric properties.

In its physical form, Acid Black 1 is typically a black powder. It exhibits good solubility in water, a critical property for its application in aqueous dyeing baths and biological staining solutions. Its absorption spectrum shows a maximum (λmax) around 618 nm, contributing to its characteristic black color. The typical properties reported include a strength of around 101.1%, low insolubles (e.g., 0.12%), and a moisture content that is carefully controlled during manufacturing.

For researchers employing Acid Black 1 in electrophoresis, its ability to bind with basic amino acid residues in proteins is key. This interaction allows for the visualization of protein bands with a sensitivity that complements other common stains. The reliability of this staining method depends heavily on the purity of the dye, emphasizing why sourcing from a trusted supplier is important.

As a manufacturer, NINGBO INNO PHARMCHEM CO.,LTD. is committed to producing Acid Black 1 that meets high standards. We understand the scientific and industrial importance of this dye and work diligently to ensure its consistent quality and availability. If you are looking to purchase Acid Black 1, understanding its chemical underpinnings reinforces the value of choosing a quality product from an experienced manufacturer in China. We invite you to request a quote to explore how our Acid Black 1 can benefit your specific applications.