In the intricate world of molecular biology and biochemical research, accurate visualization of protein expression is paramount. Electrophoresis techniques, particularly SDS-PAGE and Western blotting, rely heavily on effective staining methods to reveal the separated protein bands. Among the various dyes available, Acid Black 1, also known by synonyms like Amido Black 10B, has established itself as a critical reagent for protein detection. Its unique properties make it a preferred choice for many laboratories seeking a reliable and sensitive staining solution.

The primary advantage of Acid Black 1 (CAS 1064-48-8) lies in its ability to bind strongly to proteins. This strong affinity results in distinct and well-defined stained bands after the electrophoresis process. Researchers can therefore accurately identify and quantify proteins based on their migration patterns. Compared to other common stains like Coomassie Brilliant Blue R250, Acid Black 1 offers a different sensitivity profile, often proving advantageous in specific experimental contexts. Its detection sensitivity is approximately 20% of that of Coomassie Brilliant Blue R250, meaning it can detect lower concentrations of proteins, making it ideal for analyzing low-abundance targets.

For scientists and procurement managers looking to buy Acid Black 1 for their research needs, sourcing from a reputable manufacturer is essential. The purity of the dye directly impacts the clarity and reliability of the results. Impurities can lead to background noise or uneven staining, compromising the integrity of the experiment. Therefore, choosing a supplier that guarantees high purity is a non-negotiable step.

The manufacturing process of Acid Black 1 is complex, involving precise chemical synthesis to achieve the desired molecular structure and purity. As a leading manufacturer in China, NINGBO INNO PHARMCHEM CO.,LTD. has perfected this process, ensuring consistent quality and availability. This allows laboratories worldwide to secure a dependable supply of this vital laboratory reagent. The ability to procure high-quality Acid Black 1 at a competitive price further enhances its appeal for routine laboratory use and large-scale research projects.

Beyond its biological applications, Acid Black 1 also serves as a robust industrial dye. Its applications extend to the dyeing of wool, silk, polyamide fibers, paper, leather, and even in the formulation of certain inks and cosmetic products. This dual functionality highlights the versatility of this chemical compound. However, for research applications, the emphasis remains on its performance as a biological stain. When sourcing for electrophoresis, always ensure you are obtaining a grade specifically intended for laboratory use, guaranteeing the required purity levels. If you are looking to purchase Acid Black 1 for your laboratory, engaging with experienced chemical suppliers like NINGBO INNO PHARMCHEM CO.,LTD. will ensure you receive a product that meets stringent scientific requirements, backed by reliable service and competitive pricing from a trusted manufacturer.