Acid Blue 90 (CAS 6104-58-1), also known by its Colour Index name C.I. Acid Blue 90 or laboratory designation Coomassie Brilliant Blue G-250, is a synthetic organic compound with significant applications across various scientific and industrial fields. Understanding its chemical structure, properties, and synthesis pathways is crucial for anyone looking to procure this fine chemical. As a leading chemical manufacturer and supplier, we offer high-quality Acid Blue 90 and can provide insights into its manufacturing and use.

Chemical Properties and Characteristics

Acid Blue 90 is a triphenylmethane dye, characterized by its molecular formula C47H48N3NaO7S2 and a molecular weight of approximately 854.02. In its solid form, it typically appears as a dark blue-violet-brown powder. Its melting point is recorded at around 100°C. A key characteristic is its solubility: it is soluble in water at a concentration of 1 mg/mL and also shows solubility in ethanol. The pH of its aqueous solution is around 6.4. The dye is sensitive to light, necessitating appropriate storage conditions, often at room temperature or in a cool, dry place (e.g., 2-8°C for certain grades). Its Colour Index number, 42655, places it within the broader family of acid dyes, indicating its suitability for dyeing materials like wool, silk, and nylon.

Synthesis Overview

The synthesis of Acid Blue 90 is a multi-step process typically involving the condensation of benzaldehyde with N-ethyl-N-benzyl-3-methylaniline derivatives. Following this condensation, the resulting intermediate undergoes sulfonation, followed by oxidation and reaction with 4-ethoxybenzenamine and further benzaldehyde derivatives. The precise sequence and specific reagents, such as trisulfonic acid, are carefully controlled to yield the final product with the desired purity and structure. Manufacturers focus on optimizing these steps to ensure high yields and consistent quality, which is vital when you aim to buy this chemical.

Key Applications Driving Demand

  1. Protein Staining and Quantification: The most prominent use of Acid Blue 90 is in biochemical laboratories. It forms the basis of the Bradford assay, a widely used method for determining protein concentration in biological samples. Its ability to bind to proteins and undergo a spectral shift makes it an effective colorimetric indicator. Researchers looking to buy this chemical for analytical purposes rely on its high purity for accurate results.
  2. Biochemical Research: Beyond protein assays, Acid Blue 90 is utilized as a pharmacological tool, acting as a selective inhibitor for the P2X7 purinoceptor channel. This makes it valuable in studies investigating inflammation, pain, and neurological conditions.
  3. Industrial Dyeing: As an acid dye, Acid Blue 90 is also employed in the coloration of textiles, particularly wool and silk, and in leather dyeing. Its chemical structure provides good affinity to proteinaceous materials, offering vibrant and durable colors.

Sourcing High-Quality Acid Blue 90

For laboratories and industries requiring Acid Blue 90, sourcing from a reliable manufacturer is paramount. We, as a China-based chemical supplier, are committed to providing high-purity Acid Blue 90 that meets rigorous specifications. Whether you need it for critical protein assays or industrial dyeing, our product ensures consistent performance. We offer competitive pricing for bulk purchases and ensure timely delivery. If you are interested in buying Acid Blue 90 or require further technical information, please do not hesitate to contact us for a quote and to discuss your procurement needs.