The field of molecular biology and diagnostics relies heavily on precise and sensitive detection methods. Biotinylation, a process of attaching biotin to a molecule, is a cornerstone of many of these techniques due to biotin's high affinity for avidin and streptavidin. However, to achieve the sensitivity required for modern research, specialized biotinylation reagents are essential. Biotin-Ahx-Ahx-Tyramide (CAS 851113-28-5) represents a significant advancement in this area, offering enhanced performance for researchers worldwide.

The Evolution of Biotinylation Probes

Traditional biotinylation methods often involve direct conjugation of biotin to antibodies or other biomolecules. While effective, these methods may not always provide the amplification needed for detecting low-abundance targets. Tyramide signal amplification (TSA) systems, which utilize biotinylated tyramides, offer a solution by enzymatically depositing multiple biotin molecules at the site of target binding, thereby amplifying the signal. Biotin-Ahx-Ahx-Tyramide is a refined version of these tyramide probes, featuring a tailored linker.

Biotin-Ahx-Ahx-Tyramide: Structure and Functionality

The key feature of Biotin-Ahx-Ahx-Tyramide is its extended linker, composed of two aminohexanoic acid (Ahx) units between the biotin molecule and the tyramine functional group. This longer, more flexible linker structure can improve the accessibility and enzymatic activity of the HRP conjugate used in TSA assays. The result is a more robust signal amplification, leading to enhanced detection capabilities in sensitive applications such as immunohistochemistry (IHC) and Western blotting. As a dedicated chemical and biochemical supplier, we provide this critical reagent with guaranteed high purity (>98%), ensuring consistent experimental results.

Why Choose Us as Your Supplier?

When you choose to buy Biotin-Ahx-Ahx-Tyramide from us, you are partnering with a leading manufacturer in China known for its commitment to quality and customer satisfaction. We understand the critical nature of research chemicals and strive to offer:

  • Superior Product Quality: Our Biotin-Ahx-Ahx-Tyramide is manufactured under strict quality control, ensuring high purity essential for reproducible scientific outcomes.
  • Competitive Pricing: We offer excellent price points, particularly for bulk purchases, making advanced research tools accessible to a wider range of institutions.
  • Reliable Supply: With a stable manufacturing process, we ensure a consistent supply chain, meaning you can depend on us for your ongoing research needs.
  • Technical Support: Our team is available to answer your queries regarding product application and specifications.

Applications in Research and Diagnostics

Biotin-Ahx-Ahx-Tyramide is a versatile reagent widely used in:

  • Immunohistochemistry (IHC): For enhanced visualization of cellular and tissue components.
  • Immunocytochemistry (ICC): Similar to IHC but performed on cultured cells.
  • Western Blotting: To improve the detection of low-abundance proteins.
  • Hybridization techniques: For signal amplification in situ hybridization (ISH).

Elevate your detection capabilities by incorporating Biotin-Ahx-Ahx-Tyramide into your experimental protocols. We are your trusted source for this vital biotinylation reagent. Contact us to discuss your requirements and secure a reliable supply.