While 3-Amino-9-ethylcarbazole (AEC) is perhaps most renowned for its role as a chromogenic substrate in immunohistochemistry (IHC), its biochemical utility extends to other sensitive detection assays. Its ability to produce a colored, insoluble product upon enzymatic reaction makes it a valuable tool in a variety of biochemical and immunological techniques, particularly those involving enzyme amplification.

In assays such as enzyme-linked immunosorbent spot (ELISpot) assays, which are crucial for quantifying cytokine secretion from individual immune cells, AEC serves as an excellent chromogenic substrate. When an enzyme-linked antibody binds to a target protein, the subsequent addition of AEC and its co-factors leads to the formation of visible spots corresponding to secreting cells. The precipitate formed by AEC is distinct and stable, allowing for clear visualization and accurate counting of these cytokine-producing cells. This makes it a preferred choice for researchers studying immune responses.

Furthermore, AEC can be employed in various enzyme-linked blotting techniques, including Western blots, where it helps to visualize specific protein bands after incubation with HRP-conjugated antibodies. The resulting colored bands are easily detectable and quantifiable. The availability of AEC from reliable manufacturers and suppliers ensures that laboratories have access to this important reagent for their assay development and routine testing needs. As a trusted supplier, we are dedicated to providing AEC of the highest quality, ensuring optimal sensitivity and specificity in your biochemical assays. We encourage laboratories seeking to purchase this product to reach out for competitive pricing and reliable supply chain solutions.

The consistent performance of AEC in these demanding applications highlights its importance in modern biochemical research and diagnostics. By understanding its capabilities, researchers can effectively leverage this compound to achieve more precise and sensitive detection of biological targets.