The efficient delivery of iron to cells is paramount for life, and this process is orchestrated by the intricate interaction between transferrin and its cellular receptor. Understanding transferrin receptor binding is fundamental to grasping cellular iron metabolism and its implications in health and disease. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality Holo-Transferrin that facilitates such research.

Transferrin, particularly its iron-saturated form, Holo-Transferrin, is the primary vehicle for iron transport in the bloodstream. Cells that require iron express specific receptors on their surface, most notably the transferrin receptor (TfR), also known as CD71. This receptor has a high affinity for Holo-Transferrin. The binding process is the first critical step in cellular iron uptake. Once Holo-Transferrin binds to CD71, the complex is internalized into the cell via receptor-mediated endocytosis, forming an endosome.


Inside the endosome, the acidic environment lowers the pH, which causes Holo-Transferrin to release its bound iron ions. These iron ions are then transported out of the endosome into the cytoplasm, where they can be utilized for various cellular functions or stored. The apo-transferrin (iron-free transferrin) remains bound to the receptor within the endosome until the receptor-endosome complex is recycled back to the cell surface. At the cell surface, the neutral pH allows apo-transferrin to detach from the receptor, ready to be released back into the circulation or to bind more iron.


The efficiency and regulation of transferrin receptor binding are crucial. Cells upregulate CD71 expression when iron levels are low, ensuring an adequate supply. Conversely, high iron levels lead to downregulation of the receptor. This dynamic regulation is vital for preventing both iron deficiency and iron overload within cells. Researchers utilize Holo-Transferrin in studies to investigate these mechanisms, often using it as a supplement in cell culture media or as a tool in biochemical assays.


At NINGBO INNO PHARMCHEM CO.,LTD., we offer premium Holo-Transferrin that is essential for studies focusing on transferrin receptor binding and iron metabolism. Our product's purity and consistency ensure reliable experimental outcomes. Whether you are investigating cellular iron uptake, developing iron chelators, or researching anemia, our Holo-Transferrin is an ideal reagent. To purchase Holo-Transferrin and advance your research into cellular iron dynamics, please contact NINGBO INNO PHARMCHEM CO.,LTD.