ABTS for ELISA: A Comprehensive Guide to Chromogenic Substrate Selection
In the realm of biochemical research and diagnostics, selecting the right substrate is paramount for achieving accurate and sensitive results, especially in enzyme-linked immunosorbent assays (ELISA). Among the various chromogenic substrates available, ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) stands out as a highly favored choice for many applications.
ABTS is renowned for its exceptional sensitivity when coupled with peroxidase enzymes, such as Horseradish Peroxidase (HRP). This combination facilitates a reaction that yields a soluble green end product, which can be easily quantified using a spectrophotometer, typically at a wavelength of 405 nm. This clear and measurable signal is a significant advantage for researchers performing quantitative ELISA.
One of the key benefits of using ABTS in ELISA procedures is its stability and the production of a metastable cation. This characteristic contributes to its reliable performance and makes it a preferred substrate when dealing with potentially large background signals. Unlike some other substrates, ABTS offers a distinct advantage in achieving a high signal-to-noise ratio, which is critical for detecting low-abundance analytes.
Beyond ELISA, ABTS also finds utility in other analytical applications. For instance, it can be employed as a spectrophotometric reagent for the detection of free chlorine. Furthermore, its role in measuring the antioxidant capacities of foods highlights its versatility across different scientific disciplines. The ability to purchase ABTS from a trusted supplier like NINGBO INNO PHARMCHEM CO.,LTD. ensures researchers have access to a high-quality reagent for their experiments. When looking to buy ABTS for your lab, consider its purity and the reliability of the supplier to guarantee optimal experimental outcomes. Many researchers seek to buy ABTS for its consistent performance in various biochemical assays.
Storage of ABTS is also a critical factor for maintaining its efficacy. Typically, it should be stored desiccated at 4°C and protected from light to ensure its stability and prolong its shelf life. Adhering to these storage guidelines, as provided by NINGBO INNO PHARMCHEM CO.,LTD., will help maintain the integrity of the ABTS for your ongoing research needs. Investing in quality ABTS from NINGBO INNO PHARMCHEM CO.,LTD. ensures that your ELISA and other enzymatic assays yield dependable results.
ABTS is renowned for its exceptional sensitivity when coupled with peroxidase enzymes, such as Horseradish Peroxidase (HRP). This combination facilitates a reaction that yields a soluble green end product, which can be easily quantified using a spectrophotometer, typically at a wavelength of 405 nm. This clear and measurable signal is a significant advantage for researchers performing quantitative ELISA.
One of the key benefits of using ABTS in ELISA procedures is its stability and the production of a metastable cation. This characteristic contributes to its reliable performance and makes it a preferred substrate when dealing with potentially large background signals. Unlike some other substrates, ABTS offers a distinct advantage in achieving a high signal-to-noise ratio, which is critical for detecting low-abundance analytes.
Beyond ELISA, ABTS also finds utility in other analytical applications. For instance, it can be employed as a spectrophotometric reagent for the detection of free chlorine. Furthermore, its role in measuring the antioxidant capacities of foods highlights its versatility across different scientific disciplines. The ability to purchase ABTS from a trusted supplier like NINGBO INNO PHARMCHEM CO.,LTD. ensures researchers have access to a high-quality reagent for their experiments. When looking to buy ABTS for your lab, consider its purity and the reliability of the supplier to guarantee optimal experimental outcomes. Many researchers seek to buy ABTS for its consistent performance in various biochemical assays.
Storage of ABTS is also a critical factor for maintaining its efficacy. Typically, it should be stored desiccated at 4°C and protected from light to ensure its stability and prolong its shelf life. Adhering to these storage guidelines, as provided by NINGBO INNO PHARMCHEM CO.,LTD., will help maintain the integrity of the ABTS for your ongoing research needs. Investing in quality ABTS from NINGBO INNO PHARMCHEM CO.,LTD. ensures that your ELISA and other enzymatic assays yield dependable results.
Perspectives & Insights
Quantum Pioneer 24
“This clear and measurable signal is a significant advantage for researchers performing quantitative ELISA.”
Bio Explorer X
“One of the key benefits of using ABTS in ELISA procedures is its stability and the production of a metastable cation.”
Nano Catalyst AI
“This characteristic contributes to its reliable performance and makes it a preferred substrate when dealing with potentially large background signals.”