ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) is a chemical compound that has become indispensable in many biochemical and diagnostic laboratories. Its primary role is as a highly sensitive chromogenic substrate for peroxidase enzymes, most notably Horseradish Peroxidase (HRP). The reaction catalyzed by peroxidase, in the presence of hydrogen peroxide and ABTS, produces a soluble green radical cation.

The chemical structure of ABTS, featuring benzothiazoline rings, is key to its reactivity. This structure allows it to readily form a colored product upon enzymatic oxidation. The resulting green color has a distinct absorbance maximum, typically around 405 nm, which allows for precise spectrophotometric quantification. This makes ABTS an excellent choice for quantitative enzyme immunoassays like ELISA.

Researchers frequently seek to buy ABTS because of its versatility. Beyond ELISA, it serves as a spectrophotometric reagent for detecting free chlorine and is integral in methods to measure antioxidant capacities. Its broad applicability underscores its importance as a fundamental laboratory chemical.

Sourcing ABTS from a reliable manufacturer like NINGBO INNO PHARMCHEM CO.,LTD. ensures that you receive a product of high purity and consistent quality. This is critical for obtaining reproducible experimental results. When considering your purchase, looking at the price of ABTS from various suppliers can be helpful, but prioritize quality and consistency for your critical research.

Proper storage conditions are vital for maintaining the efficacy of ABTS. It is typically stored desiccated at 4°C and protected from light. Adhering to these recommendations, often detailed by suppliers like NINGBO INNO PHARMCHEM CO.,LTD., will ensure the longevity and performance of the substrate. Whether you are conducting routine ELISA tests or exploring novel enzymatic reactions, high-quality ABTS from a trusted source like NINGBO INNO PHARMCHEM CO.,LTD. is an essential component for success.