In the dynamic field of cell biology, precise analysis of cellular processes is paramount. Among the array of essential research tools, fluorescent dyes play a critical role, enabling scientists to visualize and quantify cellular components and activities. Acridine Orange (AO) stands out as a versatile and widely used fluorescent stain, particularly recognized for its efficacy in cell cycle analysis. As a leading provider of high-quality chemical reagents, we understand the importance of reliable sourcing for critical research materials.

Acridine Orange is a metachromatic dye that exhibits different fluorescence properties when it binds to various cellular nucleic acids. Its ability to intercalate into DNA and RNA allows researchers to differentiate between them based on the emitted fluorescence. Specifically, when Acridine Orange binds to double-stranded DNA (dsDNA), it emits a green fluorescence. Conversely, when it binds to single-stranded DNA (ssDNA) or RNA, it emits a red or orange-red fluorescence. This dual-emission characteristic is the foundation of its utility in cell cycle determination.

The process of cell cycle analysis using Acridine Orange typically involves fixing cells, followed by staining with the dye. The cells are then analyzed using flow cytometry or fluorescence microscopy. The ratio of red to green fluorescence intensity provides quantitative data on the DNA content of individual cells, allowing for the classification of cells into different phases of the cell cycle: G1 (pre-replication), S (DNA synthesis), and G2/M (post-replication). This information is invaluable for understanding cell proliferation kinetics, assessing the effects of therapeutic agents on cell division, and identifying abnormalities in cell cycle progression.

Beyond cell cycle analysis, Acridine Orange also finds significant application in detecting apoptosis (programmed cell death) and assessing autophagic activity within cells. Its ability to interact with nuclear DNA also makes it useful for chromosomal banding techniques in cytogenetics. Given its broad utility, many researchers and procurement managers actively seek reliable suppliers for Acridine Orange. When looking to buy Acridine Orange base, it is crucial to partner with a reputable manufacturer that guarantees purity and consistency. As a dedicated Acridine Orange base supplier in China, we are committed to providing researchers with the high-quality reagents necessary for their groundbreaking work. We encourage inquiries for Acridine Orange base price and bulk purchasing options to meet the demands of your laboratory, ensuring that you always have access to this essential research chemical.