For researchers in fields such as molecular biology, cell biology, and diagnostics, understanding the technical nuances of common laboratory reagents is crucial. Acridine Orange (AO) is a widely utilized fluorescent dye known for its ability to stain nucleic acids. As a dedicated supplier of high-quality chemical compounds, we aim to provide researchers with essential technical information to optimize their experimental protocols.

The primary mechanism behind Acridine Orange staining lies in its fluorescent properties and its interaction with DNA and RNA. As a cationic dye, Acridine Orange intercalates into the planar structure of nucleic acid bases. When bound to double-stranded DNA (dsDNA), the dye intercalates in a relatively planar manner, leading to fluorescence emission in the green spectrum, typically around 520-530 nm. Conversely, when AO binds to single-stranded DNA (ssDNA) or RNA, particularly at higher concentrations or under specific pH conditions, it forms stacking interactions. This binding mode results in fluorescence emission in the red or orange-red spectrum, usually around 630-650 nm.

This differential fluorescence is the basis for its application in various techniques. For cell cycle analysis, cells are stained with AO, and the ratio of red fluorescence (indicating RNA content) to green fluorescence (indicating DNA content) is measured. An increased red-to-green fluorescence ratio is often observed in cells undergoing DNA replication or in certain apoptotic states. In studies of autophagy, the shift in AO fluorescence towards red emission within acidic vesicular organelles (like lysosomes) serves as an indicator of autophagic activity.

When you choose to buy Acridine Orange base from a trusted Acridine Orange base supplier in China, you are investing in a reagent that can significantly enhance your research capabilities. We offer Acridine Orange Base that adheres to strict quality standards, ensuring reliable and reproducible results for your experiments. We encourage researchers and procurement specialists to inquire about our competitive Acridine Orange base price and to explore how our products can support your scientific investigations. Partnering with a reliable manufacturer is the first step towards successful research outcomes.