In the realm of immunology and diagnostics, the ability to precisely label antibodies is fundamental. Biotin-NHS Ester serves as an indispensable tool for this process, enabling researchers to create biotinylated antibodies that are crucial for various immunoassay techniques. As a dedicated supplier, we provide high-purity Biotin-NHS Ester, empowering scientists to achieve robust and sensitive detection systems.

Biotin-NHS Ester is specifically designed to react with the primary amine groups present on antibodies, primarily on lysine residues and the N-terminus. This amine-reactive nature allows for the covalent attachment of biotin, a small molecule with a high affinity for streptavidin and avidin. The resulting biotinylated antibodies can then be detected with unparalleled sensitivity using enzyme-conjugated or fluorescently-labeled streptavidin or avidin. This amplification strategy is a cornerstone of modern immunoassays like ELISA, Western blotting, and immunohistochemistry, significantly improving signal-to-noise ratios.

The practical application of Biotin-NHS Ester in antibody labeling involves dissolving the reagent in an organic solvent, such as DMSO or DMF, due to its limited water solubility. This solution is then added to the antibody in a buffered solution at an appropriate pH (typically 8-9) to facilitate the reaction. The short spacer arm of Biotin-NHS Ester minimizes steric hindrance, ensuring that the antibody's antigen-binding site remains accessible and functional after labeling. This is critical for maintaining the antibody's specificity and affinity.

For researchers aiming to buy reliable reagents, choosing a trusted manufacturer is crucial. Our company is a premier supplier of Biotin-NHS Ester, offering a product with 99% HPLC purity. This guarantees consistent performance and minimizes the risk of non-specific binding or aberrant reactions that can occur with lower-purity reagents. We understand the demands of immunoassay development and are committed to providing the quality materials needed for reproducible results. Whether you require small quantities for experimental optimization or bulk orders for assay production, we offer competitive pricing and prompt service.

The strategic use of Biotin-NHS Ester can significantly enhance the sensitivity and performance of your immunoassays. By ensuring the quality and purity of your labeling reagent, you lay the foundation for reliable diagnostic tools and advanced research methodologies. We invite you to request a quote for our Biotin-NHS Ester and discover the difference that a high-quality, reliable supply can make to your antibody labeling projects. Partner with us to elevate your research capabilities.