For researchers in the life sciences, precise labeling of biomolecules is paramount. Biotinylation, the process of attaching biotin to a molecule, is a widely used technique owing to biotin's exceptional affinity for avidin and streptavidin. Among the various reagents available for this purpose, Biotin-NHS Ester stands out for its ease of use and efficiency, particularly for targeting primary amines. As a leading supplier in China, we aim to equip researchers with high-quality Biotin-NHS Ester to facilitate groundbreaking discoveries.

Biotin-NHS Ester is a chemically activated form of biotin. The N-hydroxysuccinimide (NHS) ester group is highly reactive towards primary amine groups (-NH2). These amine groups are abundant in biomolecules, commonly found in the side chains of lysine residues and at the N-terminus of proteins and peptides. The reaction between the NHS ester and the amine group forms a stable amide bond, effectively conjugating biotin to the target molecule. This process is typically carried out in mild alkaline conditions (pH 8-9) in aqueous or organic solvents such as DMSO or DMF, which are often required to dissolve the otherwise water-insoluble reagent.

The utility of Biotin-NHS Ester spans numerous applications in biological research. It is extensively used for labeling antibodies, which can then be detected using avidin or streptavidin conjugates for ELISA, Western blots, or immunohistochemistry. This labeling strategy enhances detection sensitivity and allows for multiplexing. Furthermore, Biotin-NHS ester can be employed for labeling proteins for affinity purification using immobilized avidin or streptavidin resins, a cornerstone technique in protein biochemistry. Its membrane-permeable nature also makes it suitable for intracellular labeling, allowing researchers to study cellular processes and protein localization within living cells.

When sourcing this critical reagent, it is essential to partner with a reputable manufacturer and supplier. We pride ourselves on offering Biotin-NHS Ester with a high purity of 99% HPLC, ensuring consistent and reproducible experimental outcomes. Our commitment to quality means you can rely on our product for your most sensitive assays and complex bioconjugation projects. Whether you are looking to buy small quantities for initial research or require bulk supply for large-scale applications, we are equipped to meet your needs with competitive pricing and efficient delivery.

To optimize your biotinylation experiments, consider the concentration of the reagent, reaction time, and pH of the buffer. Always use fresh solutions and ensure proper storage of the Biotin-NHS Ester, typically at -20°C, to maintain its reactivity. For researchers in China and globally, we are a trusted source for high-quality bioconjugation chemicals. Contact us today to get a quote for your Biotin-NHS Ester requirements and explore how our products can advance your research in protein labeling and beyond.