Bioluminescence, the production of light by living organisms, has been harnessed as a powerful research tool, and at its core lies the substrate D-Luciferin Potassium Salt (CAS 115144-35-9). This compound, when acted upon by the enzyme luciferase, generates a measurable light signal that researchers use to probe a vast array of biological phenomena. As a dedicated supplier, we recognize the critical role this reagent plays in scientific advancement.

Understanding the Bioluminescent Reaction:

The magic of bioluminescence, as performed in the lab using D-Luciferin Potassium Salt, is a chemical reaction. The firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of ATP and oxygen. This process releases energy in the form of visible light, typically around 560 nm. The intensity of this light is directly quantifiable and correlates with the presence and activity of the luciferase enzyme, or the availability of its co-factors like ATP.

Key Research Applications:

The versatility of D-Luciferin Potassium Salt makes it an indispensable reagent in modern biological research. Here are some of its most significant applications:

  • Reporter Gene Assays: This is perhaps the most widespread use. Scientists link the luciferase gene to specific promoters or genes of interest. When cells are treated with D-Luciferin Potassium Salt, the light output directly reflects the transcriptional activity of that promoter or gene. This is fundamental for studying gene regulation, cellular signaling pathways, and screening drug candidates that might modulate gene expression. If you need to buy D-Luciferin Potassium Salt for gene expression studies, purity is key.
  • ATP Quantification: Because ATP is a mandatory substrate for the luciferase reaction, D-Luciferin Potassium Salt is a vital component in assays designed to measure cellular ATP levels. This is crucial for assessing cell viability, metabolic activity, and stress responses. Detecting low ATP levels accurately requires a reliable source of high-purity D-Luciferin Potassium Salt.
  • In Vivo and In Vitro Imaging: Bioluminescence imaging (BLI) allows researchers to visualize biological processes non-invasively within living organisms or in real-time within cell cultures. By genetically engineering cells or organisms to express luciferase, and then administering D-Luciferin Potassium Salt, researchers can track tumor progression, study infection dynamics, monitor stem cell therapy, and assess drug distribution.
  • Drug Discovery and Screening: High-throughput screening (HTS) platforms often employ bioluminescent assays utilizing D-Luciferin Potassium Salt to identify potential drug candidates that interact with specific biological targets or pathways. The speed and sensitivity of these assays make them ideal for screening large compound libraries.

The Supplier's Role in Your Research:

As you plan your experiments requiring D-Luciferin Potassium Salt (CAS 115144-35-9), selecting a dependable supplier is critical. Factors like consistent purity, proper storage recommendations, and reliable supply chains are non-negotiable. When you purchase D-Luciferin Potassium Salt, especially for sensitive applications or large-scale projects, working with experienced chemical manufacturers is recommended.

We, as a dedicated chemical supplier, understand the demands of scientific research. We offer D-Luciferin Potassium Salt that meets high purity standards, ensuring reproducible results for all your bioluminescence applications. If you are looking to order D-Luciferin Potassium Salt online, we provide the quality and reliability your research deserves.