The accurate quantification of Adenosine Triphosphate (ATP) is fundamental to understanding cellular energy metabolism, viability, and numerous biological processes. For researchers seeking a highly sensitive method, the luciferase-catalyzed oxidation of D-Luciferin Potassium Salt (CAS 115144-35-9) offers a powerful bioluminescent approach. As a leading supplier of fine chemicals, we understand the critical role of high-quality reagents in achieving precise ATP measurements.

The Luminescent Pathway to ATP Measurement:

The firefly luciferase enzyme acts as a catalyst in a reaction that requires ATP as a substrate. In the presence of oxygen, luciferase oxidizes D-luciferin, releasing light. The intensity of this emitted light is directly proportional to the concentration of ATP present in the sample. This makes D-Luciferin Potassium Salt an indispensable component for any lab aiming to perform sensitive ATP assays.

Why D-Luciferin Potassium Salt is Ideal for ATP Assays:

  • High Sensitivity: The bioluminescent reaction catalyzed by luciferase is exceptionally sensitive, allowing for the detection of very low levels of ATP, often down to femtomole quantities. This is crucial for applications involving limited sample volumes or when measuring ATP in environments with low energy turnover.
  • Specificity: The luciferase system is highly specific to ATP, minimizing interference from other nucleotides or cellular components, which ensures the accuracy of your measurements.
  • Water Solubility: The potassium salt form of D-luciferin enhances its solubility in aqueous buffers, simplifying the preparation of assay reagents and ensuring uniform light emission. When you buy D-Luciferin Potassium Salt, its water solubility is a key practical advantage.
  • Ease of Use: ATP assay kits typically include all necessary components, with D-Luciferin Potassium Salt being a readily incorporated reagent. This makes it accessible for a wide range of research applications, from basic cell viability testing to high-throughput screening.

Applications in Various Fields:

The ability to reliably detect ATP using D-Luciferin Potassium Salt makes it a versatile tool across multiple scientific disciplines:

  • Cell Viability and Proliferation Assays: Measuring intracellular ATP levels is a common method to assess the metabolic activity and overall health of cells. This is vital in toxicology studies and drug efficacy testing. If your research requires robust cell viability assessment, consider sourcing high-purity D-Luciferin Potassium Salt.
  • Microbial Contamination Detection: ATP is present in all living cells, including bacteria and yeast. Detecting ATP in samples from surfaces or liquids can quickly indicate the presence of microbial contamination, essential in food safety and pharmaceutical manufacturing.
  • Bioprocess Monitoring: In fermentation or cell culture processes, ATP levels can serve as an indicator of cell health and metabolic state, allowing for optimization of growth conditions.
  • Environmental Monitoring: ATP assays can be used to assess the microbial load in water or other environmental samples.

Sourcing D-Luciferin Potassium Salt: Your Strategic Advantage

When procuring D-Luciferin Potassium Salt, particularly if you intend to buy D-Luciferin Potassium Salt in bulk, partnering with a reputable CAS 115144-35-9 manufacturer is paramount. Look for suppliers who provide detailed product specifications, Certificates of Analysis (CoA), and consistent product quality. As a leading chemical producer in China, we offer competitive D-Luciferin Potassium Salt pricing and reliable supply chains to meet your laboratory's demands.

Ensuring the purity and quality of your D-Luciferin Potassium Salt is the first step towards obtaining accurate and reproducible ATP measurements. We are committed to being your trusted supplier, providing the essential reagents that drive scientific discovery. If you are ready to order D-Luciferin Potassium Salt online, consider us for your needs.