In the field of molecular biology, preserving the integrity of RNA is a constant challenge, primarily due to the pervasive presence of RNase enzymes. Diethyl Pyrocarbonate (DEPC) stands out as a critical chemical reagent designed to combat this issue by effectively inhibiting RNase activity. Understanding the chemical properties of DEPC and its precise applications is crucial for researchers. NINGBO INNO PHARMCHEM CO.,LTD., a prominent chemical manufacturer and supplier in China, provides high-quality DEPC that researchers can trust for their vital work.

Chemical Profile of Diethyl Pyrocarbonate (DEPC)

Diethyl Pyrocarbonate, identified by its CAS number 1609-47-8, has the molecular formula C6H10O5 and a molecular weight of 162.14. It is typically supplied as a colorless, clear liquid with a characteristic odor. Its primary mode of action against RNases involves carbethoxylation, a reaction where DEPC modifies essential amino acid residues, most notably histidine, in the active site of the enzyme. This chemical alteration irreversibly inactivates the RNase, preventing it from degrading RNA molecules. The chemical's effectiveness is further enhanced when treated water is subsequently autoclaved, safely decomposing any unreacted DEPC.

Core Applications in Preserving RNA Integrity

The most significant application of DEPC is in the preparation of RNase-free water and solutions. This is fundamental for a wide array of RNA-related experiments, including:

  • RNA Extraction: Ensuring all solutions, buffers, and labware are free from RNase contamination is paramount for obtaining high yields of intact RNA.
  • Downstream Processes: DEPC-treated water is used in preparing reagents for techniques like RT-PCR, RNA sequencing, and Northern blotting, where RNA integrity is critical.
  • General Labware Treatment: Washing glassware, plasticware, pipette tips, and tubes with DEPC-treated water helps maintain an RNase-free laboratory environment.

For researchers looking to buy DEPC, ensuring the chemical's purity is vital. NINGBO INNO PHARMCHEM CO.,LTD. guarantees a minimum assay of 99%, providing confidence in its performance.

Beyond RNase Inhibition: Other Uses of DEPC

While its role in RNA protection is primary, DEPC also serves as a useful reagent in other biochemical contexts. It can be employed as a chemical modifier for histidine and tyrosine residues in proteins, aiding in structural and functional studies. Additionally, it has found application as an antimicrobial additive, contributing to sterile laboratory conditions. Researchers can inquire about the price and availability of DEPC from NINGBO INNO PHARMCHEM CO.,LTD. for these diverse applications.

Safety and Sourcing DEPC

DEPC is classified as a hazardous chemical and must be handled with extreme caution. It is essential to use it in a fume hood, wear gloves and eye protection, and follow proper disposal procedures. Its reactivity also means it should not be added directly to Tris- or HEPES-containing buffers; instead, prepare these buffers using pre-treated DEPC water. For a reliable supply of high-quality DEPC, NINGBO INNO PHARMCHEM CO.,LTD. is a trusted chemical supplier in China, committed to product excellence and customer support.

In conclusion, Diethyl Pyrocarbonate is an indispensable reagent for any research involving RNA. Its chemical properties make it highly effective at neutralizing RNase contamination, thereby safeguarding RNA integrity. By understanding its applications and sourcing it from a reputable supplier like NINGBO INNO PHARMCHEM CO.,LTD., researchers can ensure the success and reliability of their molecular biology experiments.