The integrity of RNA is fragile, making it susceptible to degradation by ubiquitous RNase enzymes. For researchers working with RNA, the preparation and correct usage of Diethyl Pyrocarbonate (DEPC)-treated water is a fundamental practice to ensure sample quality. DEPC acts as a potent RNase inhibitor, and understanding its properties, preparation, and safe handling is key to successful molecular biology experiments. NINGBO INNO PHARMCHEM CO.,LTD., a reputable chemical supplier, provides high-quality DEPC essential for these critical applications.

What is DEPC-Treated Water?

DEPC-treated water is essentially sterile water that has been treated with Diethyl Pyrocarbonate (DEPC) to inactivate any RNase enzymes present. RNases are notoriously difficult to eliminate, as they are found in the environment and can survive standard autoclaving. DEPC (CAS 1609-47-8) works by chemically modifying RNase enzymes, rendering them inactive. The subsequent autoclaving step decomposes any residual DEPC, leaving behind safe and effective RNase-free water. This treated water is crucial for preparing solutions and washing labware used in RNA extraction, cDNA synthesis, and other RNA-related procedures.

Preparing DEPC-Treated Water: A Practical Guide

The preparation of DEPC-treated water is a standard laboratory procedure:

  1. Begin with distilled or deionized water.
  2. Add DEPC to the water at a final concentration of 0.1% (v/v). Ensure thorough mixing.
  3. Incubate the mixture at room temperature for at least 2 hours, or at 37°C for 1-2 hours. This allows DEPC to react with RNases.
  4. Autoclave the solution to decompose any remaining DEPC. This process also sterilizes the water.
  5. Allow the water to cool to room temperature before use. Store in sterile, RNase-free containers.

It is important to note that DEPC can react with certain buffers like Tris. Therefore, it's best to prepare such buffers using DEPC-treated water rather than adding DEPC directly to the buffer. Researchers can buy DEPC from trusted sources like NINGBO INNO PHARMCHEM CO.,LTD. to ensure quality and efficacy.

Safety Precautions When Handling DEPC

Diethyl Pyrocarbonate is a hazardous chemical and requires careful handling. It is a suspected carcinogen and irritant. Always work with DEPC in a well-ventilated fume hood, wearing appropriate personal protective equipment, including nitrile gloves, lab coats, and safety goggles. Avoid inhalation of vapors and skin contact. After treatment, the autoclaving step neutralizes DEPC, but proper disposal of concentrated DEPC should follow laboratory safety guidelines. NINGBO INNO PHARMCHEM CO.,LTD. provides detailed safety information with its products.

Why Choose NINGBO INNO PHARMCHEM CO.,LTD. for DEPC?

As a leading chemical manufacturer and supplier in China, NINGBO INNO PHARMCHEM CO.,LTD. offers high-purity Diethyl Pyrocarbonate (99% minimum assay) that meets the stringent requirements of molecular biology research. By choosing our product, you ensure effective RNase inactivation and reliable experimental results. We provide competitive pricing and consistent supply, making us an ideal partner for your laboratory's chemical needs.

In summary, mastering the preparation and use of DEPC-treated water is fundamental for successful RNA research. By following proper protocols and prioritizing safety, researchers can effectively eliminate RNase contamination. For your DEPC requirements, NINGBO INNO PHARMCHEM CO.,LTD. is your reliable partner, ensuring you receive a quality product for all your molecular biology applications.