Investigating the intricate world of protein-protein interactions (PPIs) is fundamental to understanding cellular processes, disease mechanisms, and developing targeted therapies. Researchers rely on specialized tools to capture and study these dynamic interactions, and homobifunctional crosslinkers like Disuccinimidyl Glutarate (DSG) are indispensable in this endeavor. As a leading supplier of high-quality biochemicals, we offer DSG to empower your research in PPIs.

What is Disuccinimidyl Glutarate (DSG)?

Disuccinimidyl Glutarate, identified by CAS 79642-50-5, is a heterobifunctional crosslinking agent. It possesses two N-hydroxysuccinimide (NHS) ester groups that readily react with primary amines on proteins. This reaction, typically performed at a pH range of 7-9, results in the formation of a stable amide bond, effectively linking two molecules together. The five-atom spacer arm within DSG provides a specific distance between the coupled entities, which can be advantageous for maintaining the structural integrity and functional activity of the interacting proteins.

Applications in Studying Protein-Protein Interactions:

DSG's ability to covalently link proteins makes it a valuable tool for a variety of research applications aimed at understanding PPIs:

  • Stabilizing Transient Interactions: Many PPIs are transient or weak. DSG can be used to 'capture' these interactions at a specific moment, allowing for their subsequent study using techniques like Western blotting, mass spectrometry, or co-immunoprecipitation. When you buy DSG, you invest in a tool to reveal these fleeting molecular partnerships.
  • Studying Protein Complexes: In the formation of larger protein complexes, DSG can help identify interacting partners and elucidate the stoichiometry and architecture of these assemblies. Our product is a reliable choice for researchers seeking to purchase high-purity crosslinkers for complex analysis.
  • Intracellular Protein Dynamics: DSG's membrane permeability allows it to be used for crosslinking proteins within living cells. This is crucial for studying dynamic PPIs that occur in specific cellular compartments or under particular physiological conditions. We are a trusted manufacturer supplying DSG for such advanced applications.
  • Protein Immobilization: For applications like enzyme immobilization on solid supports or the creation of protein arrays, DSG can covalently attach proteins, ensuring their stable attachment and functionality.

The Advantage of a Reliable Supplier

The success of PPI studies often depends on the quality and consistency of the reagents used. Sourcing Disuccinimidyl Glutarate from a reputable supplier is paramount. Key considerations include:

  • Purity: High purity (typically >97%) ensures specific amine reactivity and minimizes side reactions, leading to cleaner results and more reliable data.
  • Consistency: Batch-to-batch consistency from a reliable manufacturer guarantees that experimental conditions can be reproduced over time.
  • Availability: A stable supply chain ensures that you can readily purchase DSG when needed for critical experiments.

We are committed to being your premier source for high-quality biochemical reagents. Our Disuccinimidyl Glutarate is produced under strict quality control standards, making it an ideal choice for researchers who demand accuracy and reproducibility. Whether you are investigating molecular mechanisms or developing novel diagnostic tools, our DSG can be a critical component.

By choosing our Disuccinimidyl Glutarate, you are selecting a product backed by expertise and a commitment to scientific advancement. For those looking to buy DSG, we offer competitive pricing and dependable service. Contact us today to learn more about our product offerings and how we can support your research into protein-protein interactions.