DTT FAQs: Your Questions Answered by a Dithiothreitol Expert
Dithiothreitol (DTT) is a widely used biochemical reagent, but like any specialized chemical, it often prompts questions from users. As a dedicated DTT manufacturer and supplier, we've compiled answers to some of the most frequently asked questions to help you utilize this powerful reducing agent effectively.
What is DTT and what is its primary function?
Dithiothreitol (DTT) is a small organic molecule containing two thiol groups. Its main function is as a potent reducing agent, particularly effective at cleaving disulfide bonds (-S-S-) in proteins and preventing the oxidation of free thiol groups. This makes it invaluable for maintaining protein structure, aiding in protein purification, and protecting thiolated DNA.
What is the CAS number and molecular weight of DTT?
The CAS number for Dithiothreitol is 3483-12-3. Its molecular weight is approximately 154.25 g/mol. These details are crucial for accurate identification and stoichiometric calculations when you buy DTT.
What is the recommended assay or purity for DTT?
For most biochemical and molecular biology applications, a DTT purity of 98% or higher is recommended. Our DTT is manufactured to meet these stringent purity standards, ensuring reliable performance in your experiments. We provide detailed Certificates of Analysis with each batch.
How should DTT be stored?
DTT is hygroscopic and can be oxidized by air. It is best stored in a cool, dry place, preferably at -20°C, in a tightly sealed container. Solutions of DTT are typically prepared fresh or stored aliquoted at -20°C for short periods, as they can degrade over time.
Where can I buy DTT?
You can purchase high-quality DTT from reliable manufacturers and suppliers. We offer DTT for sale directly from our manufacturing facilities in China, providing competitive pricing and consistent quality for researchers and industrial users worldwide. Contact us for a quote or to discuss your specific DTT purchase requirements.
Perspectives & Insights
Alpha Spark Labs
“Dithiothreitol (DTT) is a widely used biochemical reagent, but like any specialized chemical, it often prompts questions from users.”
Future Pioneer 88
“As a dedicated DTT manufacturer and supplier, we've compiled answers to some of the most frequently asked questions to help you utilize this powerful reducing agent effectively.”
Core Explorer Pro
“Its main function is as a potent reducing agent, particularly effective at cleaving disulfide bonds (-S-S-) in proteins and preventing the oxidation of free thiol groups.”