Ethylene Glycol-bis(succinic acid N-hydroxysuccinimide ester), or EGS, is a critical homobifunctional crosslinker widely utilized in protein chemistry. Its distinct structure, featuring two NHS ester groups connected by a cleavable ethylene glycol spacer, makes it an invaluable reagent for researchers. Ningbo Inno Pharmchem Co., Ltd. provides EGS, ensuring high purity and reliability for demanding scientific applications. Understanding the properties of EGS is key to its effective utilization in various experimental setups, whether for protein stabilization or complex bioconjugation.

The primary function of EGS is to covalently link molecules containing primary amine groups. The reaction proceeds via NHS ester activation, forming stable amide bonds. This process is typically carried out at a neutral pH range (6.5-8.5), making it suitable for sensitive biological molecules. The 12-atom spacer arm of EGS offers a considerable length between crosslinked sites, which is advantageous in preventing steric hindrance and maintaining the conformational integrity of proteins. Researchers often seek to buy EGS for its predictable reactivity and the versatility it brings to experimental design.

In protein chemistry, EGS is employed for several purposes. It can stabilize protein structures by introducing crosslinks between specific amino acid residues, which is useful in structural studies and in enhancing the thermostability of enzymes. Furthermore, EGS is a key component in the synthesis of Antibody-Drug Conjugates (ADCs). In ADCs, a cytotoxic drug is linked to an antibody that targets cancer cells. EGS can be used as a linker to attach the drug payload to the antibody, ensuring targeted delivery and reducing systemic toxicity. The ability to buy EGS for such complex synthesis pathways highlights its importance in pharmaceutical research.

The cleavable nature of the ethylene glycol spacer in EGS adds another layer of functionality. This feature allows for the dissociation of conjugated components under specific chemical conditions, typically using hydroxylamine. This controlled cleavage is essential in various therapeutic and diagnostic applications, where the release of a drug or the separation of labeled molecules is required. When considering purchasing EGS, understanding this cleavable characteristic can unlock advanced research possibilities.

Ningbo Inno Pharmchem Co., Ltd. is committed to supporting the scientific community by providing high-quality chemical reagents. Our EGS is manufactured under strict quality control measures to ensure consistency and performance. Whether you need EGS for basic research, drug development, or diagnostic assay development, our company is a reliable source. We strive to make the purchasing process seamless, offering competitive prices and expert technical support to assist you in your scientific endeavors.