Enhancing HPLC Separations: The Role of Sodium 1-Octanesulfonate
In the realm of analytical chemistry, achieving precise and reproducible separations is paramount. High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique for separating and quantifying complex mixtures, particularly in the pharmaceutical and biochemical industries. Within HPLC, the mobile phase composition plays a critical role, and the addition of specific reagents can dramatically enhance separation efficiency. One such vital component for peptide and protein analysis is Sodium 1-Octanesulfonate (CAS 5324-84-5).
As a leading manufacturer and supplier of specialty chemicals, we understand the critical need for high-purity reagents. Sodium 1-Octanesulfonate, available with an assay of ≥98.0%, is specifically recognized for its efficacy as an ion-pairing agent. In reversed-phase HPLC, peptides and proteins often exhibit poor retention or peak shape due to their polar nature. By introducing Sodium 1-Octanesulfonate into the mobile phase, its anionic sulfonate group pairs with positively charged sites on the analyte molecules. This ion-pairing forms a more neutral, hydrophobic complex that interacts more favorably with the non-polar stationary phase. Consequently, this leads to improved retention, sharper peaks, and better resolution, enabling researchers to more accurately analyze complex biological samples.
Beyond its primary role in HPLC, Sodium 1-Octanesulfonate also functions as a surfactant. Surfactants, or surface-active agents, are compounds that lower the surface tension of liquids or the interfacial tension between two liquids, a gas and a liquid, or a liquid and a solid. This property makes them invaluable in various chemical processes, from formulations to cleaning applications. For procurement professionals seeking reliable sources, buying Sodium 1-Octanesulfonate from a reputable manufacturer in China ensures both quality and cost-effectiveness. We pride ourselves on being a premier supplier, offering competitive prices for this essential chemical intermediate.
The applications of Sodium 1-Octanesulfonate are not limited to analytical chemistry. It also serves as an intermediate in the preparation of functional materials, such as antistatic polyester fibers, highlighting its broader utility in chemical manufacturing. For those looking to purchase this compound, understanding its benefits – high purity, effective ion-pairing capabilities, and surfactant properties – is key. As a dedicated supplier, we are committed to supporting your research and industrial needs with consistent quality and responsive service. Contact us today to inquire about pricing and availability for your bulk purchase requirements.
Perspectives & Insights
Silicon Analyst 88
“In reversed-phase HPLC, peptides and proteins often exhibit poor retention or peak shape due to their polar nature.”
Quantum Seeker Pro
“By introducing Sodium 1-Octanesulfonate into the mobile phase, its anionic sulfonate group pairs with positively charged sites on the analyte molecules.”
Bio Reader 7
“This ion-pairing forms a more neutral, hydrophobic complex that interacts more favorably with the non-polar stationary phase.”