The meticulous analysis of proteins and peptides is fundamental to advancements in biotechnology, pharmaceuticals, and diagnostics. High-Performance Liquid Chromatography (HPLC) is a powerhouse technique enabling the precise separation and identification of these complex biomolecules. Central to the success of many HPLC methods, particularly in reversed-phase chromatography, is the strategic use of mobile phase additives. Sodium 1-Octanesulfonate (CAS 5324-84-5) stands out as a key reagent in this domain, primarily due to its effectiveness as an ion-pairing agent.

Proteins and peptides, being largely polar and often carrying charges at physiological or chromatographic pH, can exhibit challenging retention behavior on non-polar stationary phases typically used in reversed-phase HPLC. This is where Sodium 1-Octanesulfonate plays a crucial role. As an anionic surfactant, it forms ion pairs with positively charged sites on the protein or peptide molecules. This dynamic interaction effectively masks the charge, increasing the overall hydrophobicity of the analyte-ion pair complex. The resulting complex then interacts more strongly with the hydrophobic stationary phase, leading to enhanced retention and significantly improved separation efficiency. Researchers often rely on this reagent to achieve baseline resolution for closely related protein or peptide species, making it an indispensable component in analytical workflows.

For scientists and laboratory managers, securing a consistent supply of high-purity Sodium 1-Octanesulfonate is critical. Our company, as a dedicated manufacturer and supplier from China, ensures that our product meets rigorous specifications, typically with an assay of ≥98.0%. This high purity minimizes the introduction of unwanted side-peaks or background noise that could interfere with sensitive analyses. Beyond its role in HPLC, its surfactant properties also find utility in other biochemical processes and formulations. When you need to buy Sodium 1-Octanesulfonate for your laboratory, choosing a reputable supplier is key to ensuring the integrity of your research results.

We are committed to supporting the scientific community by providing high-quality chemical reagents. If your work involves protein or peptide analysis, or requires a reliable surfactant or chemical intermediate, we invite you to explore our offerings. Understanding the scientific principles behind its application—from ion-pairing mechanisms to surfactant behavior—reinforces the value of this compound. For bulk orders or specific purity requirements, please contact us to receive a competitive quote and discuss how we can best serve your laboratory's needs. Partner with us for your chemical supply requirements.