Guanidine Thiocyanate: Essential for Nuclease-Free DNA/RNA Isolation
The success of genetic research and molecular diagnostics hinges on the integrity of DNA and RNA. Degradation by endogenous nucleases is a persistent challenge, making the role of specific reagents critical. Guanidine Thiocyanate (GTC), identified by CAS number 593-84-0, is a premier chemical solution that offers robust protection against these enzymatic threats. As a leading Guanidine Thiocyanate supplier in China, NINGBO INNO PHARMCHEM CO.,LTD. provides a high-purity product essential for nuclease-free nucleic acid isolation.
Guanidine Thiocyanate functions as a potent chaotropic agent. Its primary mechanism in nucleic acid isolation is its ability to denature proteins, including RNases and DNases, which are enzymes that can rapidly degrade RNA and DNA, respectively. By unfolding these enzymes, GTC renders them inactive, thereby preserving the integrity of the genetic material during the extraction process. This property makes GTC an indispensable component in lysis buffers and extraction kits designed for obtaining high-quality DNA and RNA.
The typical process involves using GTC in a buffered solution to lyse cells and viruses. This action not only releases the nucleic acids but also immediately neutralizes any nucleases present. The effectiveness of GTC ensures that researchers can achieve higher yields of intact DNA and RNA, which are crucial for downstream applications such as PCR, quantitative PCR (qPCR), sequencing, and gene expression analysis. A high-purity Guanidine Thiocyanate supplier is key to ensuring these processes are not compromised by reagent impurities.
When considering where to buy Guanidine Thiocyanate, it is important to look for a supplier that guarantees consistent purity and quality. Our company, as a dedicated Guanidine Thiocyanate manufacturer in China, emphasizes product excellence. We provide Guanidine Thiocyanate with a typical purity of ≥99.5%, ensuring it is suitable for even the most sensitive molecular biology applications. Understanding the Guanidine Thiocyanate price in bulk can also aid in efficient budgeting for research projects and commercial production.
The stability of Guanidine Thiocyanate itself is also a factor; it is generally stable but light-sensitive, requiring appropriate storage. For laboratories and manufacturers requiring a steady supply, partnering with a reliable Guanidine Thiocyanate manufacturer like NINGBO INNO PHARMCHEM CO.,LTD. is crucial. We are committed to supporting the scientific community by offering a dependable source of this vital biochemical reagent, ensuring your work in genomics and molecular diagnostics proceeds without interruption from enzymatic degradation.
For researchers and businesses seeking a trusted source to buy Guanidine Thiocyanate for nuclease-free isolation, we welcome your inquiries. Our team is ready to provide detailed product specifications, competitive pricing for bulk orders, and efficient logistical support. Secure your supply chain with high-quality Guanidine Thiocyanate from a premier China-based supplier and ensure the integrity of your valuable nucleic acid samples.
Perspectives & Insights
Future Origin 2025
“provides a high-purity product essential for nuclease-free nucleic acid isolation.”
Core Analyst 01
“Its primary mechanism in nucleic acid isolation is its ability to denature proteins, including RNases and DNases, which are enzymes that can rapidly degrade RNA and DNA, respectively.”
Silicon Seeker One
“By unfolding these enzymes, GTC renders them inactive, thereby preserving the integrity of the genetic material during the extraction process.”