For chemists and formulation scientists, understanding the precise chemical properties of a compound is the first step toward successful application. HEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid), identified by CAS number 7365-45-9, is a prime example of a specialty chemical that offers remarkable utility across various scientific disciplines. As a dedicated manufacturer and supplier, we aim to provide comprehensive insights into this vital biological buffer.

Chemically, HEPES is characterized by its zwitterionic nature, featuring both a positively charged piperazine ring and a negatively charged sulfonate group within the same molecule. This unique structure contributes to its high water solubility and its ability to remain largely un-ionized across a broad pH range. The critical functional aspect of HEPES is its pKa, reported to be between 7.4 and 7.7 at 20°C. This means HEPES is most effective as a buffer when operating within this specific pH range, which closely mirrors the physiological conditions found within living organisms. This makes it an indispensable component in many biochemical and biological research applications where maintaining a stable pH is crucial for enzyme activity, protein stability, and cellular integrity.

The stability of HEPES is another key attribute. It is resistant to enzymatic degradation and exhibits minimal interaction with metal ions, which can be problematic with other common buffers. This inertness ensures that HEPES does not interfere with the biological or chemical reactions being studied, a critical factor for reliable experimental results. Researchers often seek out high-purity HEPES, typically with an assay of 99.5% or higher, to avoid confounding variables that impurities might introduce. As a manufacturer committed to quality, we supply HEPES in a consistent, free-flowing white powder form, simplifying its integration into diverse laboratory protocols.

From a chemical sourcing perspective, procurement managers and research scientists often look for reliable suppliers in China. We understand the importance of consistent quality and competitive pricing for chemicals like HEPES. Whether your work involves in vitro diagnostics, protein crystallization, or sophisticated cell culture media, our company is positioned to be your trusted HEPES manufacturer. We offer not just the product but also the assurance of stringent quality control and a responsive supply chain.

The applications of HEPES are widespread, extending beyond basic cell culture. It is frequently employed in electrophoresis buffers, where its buffering capacity helps maintain uniform ion environments. In protein chemistry, it's used in buffer systems for enzyme assays, protein isolation, and structural studies. For scientists working with nucleic acids, HEPES can be part of buffer formulations for DNA/RNA manipulation and storage. Its utility in maintaining pH stability is fundamental to achieving reproducible results in these complex chemical and biological processes.

If you are looking to buy HEPES and require a dependable supplier, consider our offerings. We provide detailed specifications, including assay results and physical properties, to ensure you receive the exact material for your application. Engaging with us means partnering with a manufacturer dedicated to supporting your scientific endeavors with high-quality HEPES and excellent customer service. Contact us to inquire about purchasing options and to secure a consistent supply of this essential chemical reagent.